麦角硫因对D-半乳糖诱导大鼠氧化应激损伤的抗氧化活性研究OA

This study aimed to evaluate the protective effect of ergothioneine(ERGO)on D-galactose(D-gal)-induced oxidative stress damage in rats and to explore its potential mechanisms.SD male rats were used as research subjects and were modeled by intraperito-neal injection of D-gal solution.They were randomly divided into five groups,including a blank group,a model group,an ERGO low-dose group(ERGO-L,1.25 mg/kg),an ERGO medium-dose group(ERGO-M,2.5 mg/kg),and an ERGO high-dose group(ERGO-H,5.0 mg/kg).Rats received corresponding administrations once daily for 4 weeks.The levels of malondialdehyde(MDA),protein carbonyl(PC),superoxide dismutase(SOD),catalase(CAT),and reduced glutathione(GSH)in rat serum,liver,kidney,and spleen were measured by colorimetry.The composition of the gut microbiota and the content of short-chain fatty acids(SCFAs)in each group of rats were analyzed by 16S sequencing and ultra-high-performance liquid chromatography-tandem triple quadrupole mass spectrometry to evalu-ate the potential antioxidant mechanism.Results showed that compared with the model group,the administration groups significantly re-duced the levels of MDA and PC in rat serum,liver,kidney,and spleen to varying degrees(P＜0.05 or P＜0.01)and significantly in-creased the activities of SOD and CAT and the level of GSH(P＜0.05 or P＜0.01).Except for the low-dose group,which had no signifi-cant reduction in IL-1β levels,all other dose groups significantly reduced the levels of IL-1β,IL-6,and TNF-α in the serum of rats with oxidative stress damage(P＜0.05 or P＜0.01).Compared with the model group,the contents of short-chain fatty acids(SCFAs),inclu-ding butyrate,isobutyrate,and valerate,were significantly decreased in the gut of rats with oxidative damage(P＜0.01).Compared with the model group,ERGO significantly increased the SCFA contents in the gut of rats with oxidative damage.ERGO administration has a sig-nificant protective effect on D-gal-induced oxidative damage in rats,and its mechanism may be achieved by reducing inflammation,regula-ting the gut microbiota to promote the generation of short-chain fatty acids,and direct antioxidant effects.