海带寡糖对果蝇抗氧化酶活性及抗衰老作用研究OA
Effects of Laminaria japonica Oligosaccharides on Antioxidant Enzyme Activities and Anti-aging in Drosophila melanogaster
目的:研究海带寡糖对果蝇抗氧化酶及抗衰老作用.方法:采用野生型残翅果蝇为实验对象,将海带寡糖LOs2(Laminaria japonica oligosaccharides 2)按低、中、高剂量(分别为 0.25%、0.50%、1.00%)添加至培养基中,通过测定果蝇体内超氧化物歧化酶(Superoxide dismutase,SOD)活力、丙二醛(Malonic dialdehyde,MDA)含量、过氧化氢酶(Catalase,CAT)活力以及果蝇寿命实验,评价海带寡糖的抗氧化及抗衰老作用.结果:与空白对照组相比,总抗氧化能力(T-AOC)在 1.00%剂量组表现最佳,达到 45.87%(雌果蝇)和76.21%(雄果蝇);1.00%剂量组显著降低了 MDA 含量(P<0.05),雌、雄果蝇分别降低了 82.77%和 61.24%;LOs2在 1.00%剂量组能显著提高雌、雄果蝇 SOD 和 CAT 的活性(P<0.05);饲喂 LOs2的 1.00%剂量组果蝇较空白对照组,最高寿命指标显示,雌性延长 54.13%,雄性延长 72.00%;LOs2在 1.00%剂量组,SOD1、SOD2、CAT 的基因表达量均显著上调(P<0.05).结论:LOs2可能通过上调 SOD1、SOD2和 CAT基因的表达,减少果蝇体内过量的活性氧和丙二醛,从而延缓衰老并延长其寿命.海带寡糖具有较好的体内外抗氧化作用和潜在抗衰老作用.
Objective:To evaluate the antioxidant capacity and anti-aging potential of Laminaria japonica oligosaccharides 2(LOs2)in Drosophila melanogaster.Method:Wild-type vestigial-wing D.melanogaster were reared on standard medium supplemented with LOs2 at low(0.25%,w/w),medium(0.50%),or high(1.00%)doses.The antioxidant and anti-aging effects of LOs2 were evaluated by determining the activity of superoxide dismutase(SOD),the content of malonic dialdehyde(MDA),the activity of catalase(CAT)in D.melanogaster,and the lifespan experiment of D.melanogaster.Result:Relative to the blank control,the 1.00%LOs2 group showed the highest T-AOC,increasing by 45.87%in females and 76.21%in males.MDA levels were markedly reduced(82.77%in females,61.24%in males)(P<0.05).SOD and CAT activities rose significantly at 1.00%LOs2 in both sexes(P<0.05).The 1.00%LOs2 group showed a 54.13%increase in female lifespan and a 72.00%increase in male lifespan compared to the blank control group.Consistently,qPCR revealed marked up-regulation of SOD1,SOD2,and CAT in the 1.00%group(P<0.05).Conclusion:These findings suggested that LOs2 attenuated the accumulation of excessive ROS and MDA by up-regulating the expression of SOD1,SOD2,and CAT genes,thereby delaying aging of D.melanogaster.LOs2 exhibited favorable antioxidant activity in vitro and in vivo,as well as potential anti-aging effects.
王蕾;邱佳怡;周知锦;陈甜怡;谢颖星;蔡旭正;汪财生;钱国英
浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100浙江万里学院生物与环境学院,浙江 宁波 315100
轻工纺织
海带寡糖抗氧化抗衰老果蝇
Laminaria japonica oligosaccharidesantioxidantanti-agingDrosophila melanogaster
《食品工业科技》 2026 (9)
421-429,9
宁波余姚市农社科类科技创新项目(2025JH03010017).
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