基于磁感应电场-酶解耦合技术的鲍鱼内脏牛磺酸提取、纯化及表征OA

This study established a novel magnetically induced electric field-enzymatic hydrolysis(MIEF-EH)coupled technology for the sustainable taurine extraction from abalone viscera byproducts.The results of the study indicated that the optimal extraction conditions were as follows:an excitation voltage of 1400 V,an electric field frequency of 50 kHz,a flow rate of 25 L/h,and a reaction time of 1 h.Under the optimal conditions,MIEF-EH achieved a taurine yield of 12.36 mg/g,significantly(P<0.05)outperforming both individual methods(such as MIEFE,microwave,enzymatic hydrolysis and hydrothermal)as well as other coupled approaches(microwave-enzymatic hydrolysis and hydrothermal-enzymatic hydrolysis).SEM revealed pronounced particle-size reduction and the formation of a loose,porous microstructure,correlating with improved taurine extraction.Subsequent purification via ion-exchange resin yielded taurine with 92.29%recovery and 96.78%purity,while ethanol precipitation achieved 95.30%recovery and 94.43%purity.The chemical structure and molecular mass of the purified taurine were confirmed through multi-technique validation(FT-IR,MS,and 1H NMR).All physicochemical properties complied with the specifications of the Chinese National Standard for Food Additive Taurine(GB 14759-2010).In summary,the MIEF-EH coupled technology developed in this study provides a feasible technological pathway for green conversion of abalone viscera waste into high-purity natural taurine.