基于CRISPR/Cas技术的肉类掺假检测研究进展OA
Advances in Meat Adulteration Detection Based on CRISPR/Cas Technology
近年来,肉类掺假问题日益突出,不仅威胁食品安全,还涉及宗教信仰、经济利益等多个层面,已成为亟待解决的公共安全问题.尽管现有多种肉类掺假检测方法,但传统技术仍难以满足现场快速、精准检测需求,因此建立快速、准确的现场检测方法意义重大.成簇规则间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及其相关蛋白(CRISPR-associated proteins,Cas)组成的CRISPR/Cas系统因其反式切割活性和高特异性优势,已成为一种超灵敏核酸检测工具,并在肉类掺假检测领域展示出巨大潜力.本文总结现有肉类掺假检测方法的优缺点,阐述CRISPR/Cas系统的组成、分类及作用机制,重点探讨基于CRISPR/Cas技术的肉类掺假检测应用,并分析该技术的优势、挑战及未来发展方向.
In recent years,meat adulteration has become a prominent issue threatening food safety and touching on religion,economy,and more,making it a pressing social problem.While various detection methods exist for meat adulteration,traditional techniques fall short in meeting the demand for rapid and accurate on-site detection.Thus,developing rapid and accurate methods for on-site detection of meat adulteration is of great significance.The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system,composed of CRISPR and Cas,has the advantages of trans-cleavage activity and high specificity and has become an ultrasensitive nucleic acid detection tool.It shows significant potential in meat adulteration detection.This paper reviews the pros and cons of the existing methods for meat adulteration detection,and introduces the composition,classification,and mechanism of action of the CRISPR/Cas system,with a focus on the application of CRISPR/Cas-based technology in meat adulteration detection.It also discusses the advantages,challenges and future directions of this technology.
郭润泽;张颖;杨瑞琴;孙启凡
中国人民公安大学侦查学院,北京 100038公安部鉴定中心,北京 100038中国人民公安大学侦查学院,北京 100038公安部鉴定中心,北京 100038
轻工纺织
成簇规则间隔短回文重复序列及其相关蛋白核酸检测肉类掺假
clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteinsnucleic acid detectionmeat adulteration
《肉类研究》 2026 (7)
73-80,8
"十四五"国家重点研发计划重点专项(2022YFC3341002)
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