枸杞多糖的分离纯化、结构分析及抗神经炎症活性OA

Lycium barbarum polysaccharides(LBPs)are the predominant bioactive compounds in Lycium barbarum and exhibit multiple biological activities.To explore their potential anti-neuroinflammatory effects,we screened for an optimal decolorization resin and ultrafiltration membrane by using decolorization rate,polysaccharide recovery rate,and separation time as evaluation indices.A novel method for isolating and purifying LBPs was developed by coupling macroporous ion-exchange resin chromatography with ultrafiltration membrane technology.Through this approach,LBPs-N was successfully obtained.LBPs-T were prepared through conventional water extraction/alcohol precipitation and hydrogen peroxide-induced oxidative decolorization.The composition and structural characteristics of both LBPs-N and LBPs-T were analyzed using high-performance gel permeation chromatography and pre-column derivatization high-performance liquid chromatography.Their anti-neuroinflammatory activities were evaluated via the Griess method and ELISA.LX-360 resin provided optimal decolorization performance,achieving respective decolorization rates and polysaccharide recoveries of 85.64%and 80.83%.The optimum ultrafiltration membrane molecular weight was 5.0 kDa,with a polysaccharide recovery of 73.54%.LBPs-N is primarily composed of glucose,galactose,and arabinose,whereas LBPs-T predominantly consist of galactose and arabinose.Although LBPs-N and LBPs-T exhibit distinct differences in polysaccharide purity,molecular weight distribution,and triple-helix conformation,both fractions significantly suppress pro-inflammatory cytokine secretion,thereby alleviating lipopolysaccharide-induced microglial inflammation.Notably,LBPs-N demonstrated stronger inhibition of TNF-α,IL-6,and IL-1β.The novel method for isolating and purifying LBPs established in this study provides a theoretical foundation for their potential application in mitigating neurodegenerative diseases.