转录因子PdTP1在指状青霉中调控α-松油醇合成及菌株生长的分子机制OA
Molecular Mechanism of the Transcription Factor PdTP1 in Regulating α-Terpineol Biosynthesis and Fungal Growth in Penicillium digitatum
为了完善 Zn2Cys6 型转录因子 PdTP1 的调控网络,该研究通过转录组测序技术筛选与指状青霉(Penicillium digitatum)DSM 62840 生物转化柠檬烯生成 α-松油醇及调控菌株生长相关的基因,挖掘相关通路.该研究通过对野生型菌株 DSM 62840 和 PdTP1 沉默菌株进行转录组测序来探究差异表达基因的主要代谢途径及生物学功能,并结合荧光定量PCR验证转录组测序结果的可靠性.结果表明,共鉴定到差异表达基因 1 221个,其中上调基因 499个,下调基因 722个,GO 和 KEGG 分析显示这些差异表达基因显著富集在糖、氨基酸、脂肪酸等物质的代谢与合成过程中.其中,一些与萜类代谢或合成、真菌生长以及应激响应相关基因的表达明显受到PdTP1 的调控,表明这些基因可能在参与柠檬烯生物转化生成 α-松油醇、调控指状青霉生长中发挥重要作用.荧光定量PCR 的实验结果也证实了转录组的可靠性.综上,该研究通过研究 PdTP1 调控基因转录水平上的变化,完善了其转录调控网络,进一步为阐明转录因子 PdTP1 在调控 α-松油醇合成及菌株生长中的分子机制提供依据.
To enhance the regulatory network of the Zn2Cys6-type transcription factor,PdTP1,this study employed transcriptome sequencing technology to screen for genes related to the biotransformation of limonene into α-terpineol by Penicillium digitatum DSM 62840 and the regulation of fungal growth.Transcriptome sequencing was performed on the wild-type P.digitatum DSM 62840 and PdTP1-silenced strains to investigate the metabolic pathways and biological functions of key differentially expressed genes(DEGs).Reliability of the transcriptome sequencing results was verified through quantitative real-time PCR.A total of 1 221 DEGs,including 499 upregulated and 722 downregulated genes,were identified.GO and KEGG analyses indicated that these DEGs were significantly enriched in the metabolism and biosynthesis of various substances,including sugars,amino acids,and fatty acids.Among them,the expression of certain genes related to terpene metabolism or synthesis,fungal growth,and the stress response were significantly regulated by PdTP1,suggesting that these genes may play important roles in regulating the biotransformation of limonene to α-terpineol and the growth of P.digitatum.Reliability of the transcriptome sequencing results was confirmed by the quantitative real-time PCR results.In summary,this study refined the regulatory network of the transcription factor,PdTP1,by examining variations in gene transcription regulated by it,providing evidence for elucidating the molecular mechanisms by which PdTP1 regulates α-terpineol biosynthesis and fungal growth.
黄佳琳;张婷;王子轩;张佳月;王婧玥;安康;程慧如;王静文;张璐璐
河南工业大学粮油食品学院,河南 郑州 450001河南工业大学粮油食品学院,河南 郑州 450001河南工业大学国际教育学院,河南 郑州 450001河南工业大学粮油食品学院,河南 郑州 450001河南工业大学国际教育学院,河南 郑州 450001河南工业大学粮油食品学院,河南 郑州 450001河南工业大学粮油食品学院,河南 郑州 450001河南工业大学粮油食品学院,河南 郑州 450001河南工业大学粮油食品学院,河南 郑州 450001
转录因子PdTP1α-松油醇菌株生长转录组基因
PdTP1α-terpineolfungal growthtranscriptomegenes
《现代食品科技》 2026 (3)
64-74,11
国家自然科学基金青年基金项目(32101868)中国博士后科学基金面上项目(2024M760795)河南省博士后启动经费资助项目(HN2025039)河南省科技研发计划联合基金(242103810080)河南工业大学高层次人才基金项目(2021BS054)
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