贝莱斯芽孢杆菌SN-1胞外多糖对人肝癌HepG2细胞的抑制作用OA

We investigated the impacts of exopolysaccharides isolated from Bacillus velezensis SN-1(referred to as rEPS-2)on human hepatocellular carcinoma HepG2 cells,including their influence on apoptosis.HepG2 cells were treated with rEPS-2 for 48 hours,and their morphological characteristics were observed using an inverted microscope and fluorescence microscopy.An MTT assay was employed to examine the effects of rEPS-2 treatment for 24 and 48 hours on HepG2 cell survival and proliferation.The impact of treatment on cell cycle distribution was analyzed by flow cytometry.The inhibitory effects of rEPS-2 on HepG2 cells were time and dose dependent.At an rEPS-2 concentration of 2 mg·mL-1,inhibition of cell proliferation reached 72.35%.Flow cytometry analysis using Annexin V/PI double staining indicated dose dependent induction of G1/S phase arrest,with an increased percentage of cells in G0/G1 phase.rEPS-2 also increased the activity of apoptosis-associated enzymes Caspase-9 and Caspase-3.After 48 hours of treatment with rEPS-2,Caspase-3 and Caspase-9 activities increased to 140%and 162%,respectively.In conclusion,rEPS-2 displays significant in vitro antiproliferative efficacy against HepG2 cells.These findings provide a reference and supportive data for rEPS-2 development and application.