西藏墨脱引起茶树叶枯病的两种镰刀菌新病原鉴定和生物学特性OA

In June and July 2024,two Fusarium spp.strains were isolated from blighted leaves of tea plants(Camellia sinensis)in Motuo County,Linzhi City,Xizang,China.Species identification was conducted using morphological characteristics,multi-locus phylogenetic analysis,and Koch's postulates.The results identified the strains as Fusarium dracaenophilum and Fusarium asiaticum.Pathogenicity tests confirm that both species induce tea leaf blight.In varietal pathogenicity assays,'Zhonghuang 1'exhibited significantly stronger resistance to both pathogens than'Fuding Dabaicha'.The results of the biological characterization reveal that F.asiaticum exhibits optimal growth conditions at temperatures ranging from 20 ℃ to 25 ℃ and at pH levels between 7 and 9.The presence of sucrose,maltose,lactose,and yeast extract has been demonstrated to stimulate F.asiaticum growth.F.dracaenophilum exhibits optimal growth at 25 ℃ and pH 7,with lactose and yeast extract being the most favorable nutrients.These findings enhanced understanding of Fusarium pathogen diversity and provided a theoretical basis for managing foliar diseases in tea plantations in Xizang.