低温等离子体诱导乳清分离蛋白-壳聚糖复合颗粒的结构特性及其花青素负载性能OA

To investigate the inducing effect of dielectric barrier discharge cold plasma(DBD-CP)technology(treatment voltages:30,90,150 kV,treatment times:30,60,90 s)on the conformational changes of whey protein isolate(WPI)-chitosan(CS)composite particles and its regulatory effect on the loading performance of anthocyanins(ACNs),this study employed a two-factor full factorial experimental design.By systematically evaluating the encapsulation efficiency(EE),loading capacity(LC),ζ-potential,and particle size distribution of the composite particles for ACNs,the optimal DBD-CP treatment parameters were screened.Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),scanning electron microscopy(SEM),fluorescence spectroscopy(FS),and circular dichroism(CD)were utilized as multi-scale characterization techniques to deeply analyze the DBD-CP-induced molecular conformational changes in WPI-CS.Based on the results of the above systematic evaluation,the optimal DBD-CP treatment parameters were screened and determined as voltage 90 kV and time 60 s.Under this optimal parameter set,the EE and LC of ACNs increased to 91.93%and 4.59%,respectively,significantly higher than those of the untreated group(P<0.05).The ζ-potential increased,enhancing electrostatic repulsion between particles and significantly improving system stability.Particle size analysis showed that distribution uniformity and particle dispersibility were improved.Structural characterization revealed that:Reactive species(e.g.,electrons,ions)in DBD-CP promoted the exposure of hydrophobic groups in WPI and decreased the deacetylation degree of CS through oxidative modification,thereby enhancing the formation of hydrogen bonding and electrostatic cross-linking networks,simultaneously,the plasma etching effect optimized the pore uniformity of the complex,forming a dense encapsulation structure.DBD-CP treatment effectively modulated the structure of WPI-CS composite particles through synergistic effects(oxidative modification induced by reactive species and the etching effect),significantly improving their loading performance for ACNs and the system stability.This provides a theoretical basis for the application of cold plasma technology in stabilized delivery systems for bioactive components.