中亚白及中性多糖的结构解析及抗炎活性研究OA

To systematically investigate the structural features and anti-inflammatory activity of neutral polysaccharide(USP-N)from Orchis chusua D.Don,a medicinal and edible plant was selected.The structure of USP-N was comprehensively elucidated through partial acid hydrolysis,Smith degradation,methylation analysis,and 2D-NMR spectroscopy.Its anti-inflammatory effects were evaluated using a zebrafish inflammation model.Structural analysis revealed that the main chain of USP-N was composed of alternating β-1,4-linked glucose(38.5%)and mannose(33.8%),forming a glucomannan backbone.The side chains contained small amounts of rhamnose,arabinose,and galactose,with some mannose residues featuring 2-O-acetyl groups.Bioactivity assays demonstrated that USP-N treatment significantly suppressed LPS-induced nitric oxide(NO)release(P<0.0001)and inhibited neutrophil migration to the tail injury site in zebrafish(P<0.0001).Furthermore,dose-dependent reductions in neutrophil and macrophage recruitment to the caudal region following mechanical injury were observed.This study provides a theoretical foundation for applying Orchis chusua D.Don polysaccharides in functional foods and pharmaceutical development.