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不同菌种发酵对牛肉酱品质的影响OA

Effect of fermentation with different strains on the quality of beef sauce

中文摘要英文摘要

该研究分别采用植物乳植杆菌(Lactiplantibacillus plantarum)、酿酒酵母(Saccharomyces cerevisiae)和米曲霉(Aspergillus oryzae)制作发酵牛肉酱.通过常规检测方法、气相色谱-质谱(GC-MS)法、电子鼻及感官评定法测定牛肉酱的pH值、质构特性、挥发性风味物质、电子鼻响应值及感官评分,考察不同发酵菌剂对牛肉酱品质的影响,并基于电子鼻响应值对各组牛肉酱进行主成分分析(PCA).结果表明,酵母菌发酵的牛肉酱pH(4.87)、硬度(74.96N)和咀嚼性(75.57 mJ)适中;米曲霉通过酶解作用使硬度和咀嚼性较低,但胶粘性下降导致质地松散,色泽暗沉;酵母发酵的牛肉酱挥发性风味物质种类最为丰富,达53种,与不加发酵剂制作的牛肉酱相比,醇类物质和酯类物质含量分别提高14.04和20.7个百分点,赋予牛肉酱独特的果香和花香;酵母菌发酵牛肉酱对醇类、醛酮类、氮氧化合物等敏感,香气特征显著优于其他组.主成分分析可基于电子鼻响应值有效区分不同菌种发酵的牛肉酱;感官分析结果表明,酵母发酵组感官评分最高,为(85.89±4.48)分.综上,酿酒酵母通过调控挥发性风味物质合成与质构优化,在提升牛肉酱综合品质方面表现最优.

Fermented beef sauce was produced using three starter cultures-Lactiplantibacillus plantarum,Saccharomyces cerevisiae,and Aspergillus oryzae.The pH,textural properties,volatile flavor compounds,electronic nose responses value,and sensory scores were determined by conventional methods,gas chromatography-mass spectrometry(GC-MS),electronic nose,and sensory evaluation,respectively.The effects of different fermentation agents on the quality of beef sauce were investigated,and principal component analysis(PCA)was conducted on each group of beef sauce based on the electronic nose response values.The results indicated that the yeast-fermented beef sauce exhibited a moderate pH of 4.87,along with appropriate hardness(74.96 N)and chewiness(75.57 mJ).A.oryzae reduced hardness and chewiness through enzymatic hydrolysis,but decreased adhesiveness led to a loose texture and darkened color.The yeast-fermented beef sauce showed the richest variety of volatile compounds(53 types).Compared with the control,the relative contents of alcohols and esters were increased by 14.04 and 20.7 percentage points,respectively,imparting unique fruity and floral aromas.The yeast-fermented beef sauce was sensitive to alcohols,aldehydes,ketones,and nitrogen oxides,and its aroma profile was significantly better than those of the other groups.PCA could effectively distinguish beef sauce fermented with different strains based on electronic nose response values.Sensory evaluation results showed that the yeast-fermented group achieved the highest sensory score(85.89±4.48).In summary,S.cerevisiae exhibited the optimal performance in enhancing the comprehensive quality of beef sauce by regulating the synthesis of volatile flavor compounds and optimizing texture.

罗爱国;胡变芳;姜佩含;陈泽刚;孔建纲;赵红梅;马晓丽;杨艳君;史胜利;刘晓夏

晋中学院 生物科学与技术系,山西 晋中 030619||晋中学院 山西省复合调味品技术创新中心,山西 晋中 030619晋中师范高等专科学校,山西 晋中 030600晋中学院 生物科学与技术系,山西 晋中 030619山西省平遥牛肉集团有限公司,山西 晋中 043000山西省平遥牛肉集团有限公司,山西 晋中 043000晋中学院 生物科学与技术系,山西 晋中 030619||晋中学院 山西省复合调味品技术创新中心,山西 晋中 030619晋中学院 生物科学与技术系,山西 晋中 030619||晋中学院 山西省复合调味品技术创新中心,山西 晋中 030619晋中学院 生物科学与技术系,山西 晋中 030619||晋中学院 山西省复合调味品技术创新中心,山西 晋中 030619晋中学院 生物科学与技术系,山西 晋中 030619||晋中学院 山西省复合调味品技术创新中心,山西 晋中 030619晋中学院 生物科学与技术系,山西 晋中 030619||晋中学院 山西省复合调味品技术创新中心,山西 晋中 030619

轻工纺织

发酵菌剂牛肉酱理化性质挥发性风味物质感官评价

fermentation agentsbeef saucephysicochemical propertyvolatile flavor compoundsensory evaluation

《中国酿造》 2026 (3)

236-243,8

山西省高等学校科技创新计划创新平台项目(2022P022)

10.11882/j.issn.0254-5071.2026.03.032

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