甜瓜蔓枯病病原菌鉴定及拮抗菌筛选OA

Gummy stem blight is one of the main diseases of cucurbit crops,which seriously affects the quality and yield of melons.To clarify the species of pathogens causing gummy stem blight in the main melon-producing areas of Xinzhou city,Shanxi province,this study carried out isolation and identification of the pathogens.Antagonistic strains were isolated and screened from the rhizosphere soil of healthy melons,the species of the strains were identified by combining 16S rRNA sequence analysis,and their biocontrol effects were evaluated through pot experiments.The results showed that the pathogen causing melon gummy stem blight in this region was Stagonosporopsis cucurbitacearum.Five strains(XJ18,XJ10,XJ2,XJ5,and XJ9)obtained by screening had good antagonistic effects against the pathogen,with inhibition rates of 63.40%,61.67%,60.40%,58.83%,and 55.14%respectively,and they were identified as B.halotolerans,B.amyloliquefaciens,B.mojavensis,B.velezensis,and Bacillus rugosusrespectively.Pot experiments showed that XJ18 and XJ2 had the most significant control effects,with control efficiencies of 72.84%and 69.43%respectively.The above results provided a reliable scientific basis for the biological control of melon gummy stem blight.