富含麦角甾醇的香菇柄提取物调节大鼠脂质代谢OA

The effects of ergosterol-rich Lentinus edodes stalk extracts on lipid metabolism remain unknown;thus,the content of ergosterol in the cap and stalk of L.edodes was analyzed using high performance liquid chromatography and the regulatory effects of the n-hexane extract(SEHE)and methanol/dichloromethane extract(SEMD)of the stalk on serum lipids,liver lipids and fecal lipids investigated in a rat model induced by a high-cholesterol diet.The ergosterol content of 7.82 mg·g-1 in the L.edodes cap was found to be significantly higher than the 5.24 mg·g-1 in the stalk,and the ergosterol content of SEHE was 20.31％(m/m)was higher than the 6.12％(m/m)obtained for SEMD.Animal experiments showed that both SEHE and SEMD could significantly reduce the level of low-density lipoprotein cholesterol(LDL-C),with reductions of 51.02％(P＜0.05)and 58.34％(P＜0.05),respectively,and effectively reduce the LDL-C/high-density lipoprotein cholesterol(HDL-C)ratio,with reductions of 54.61％(P＜0.05)and 53.04％(P＜0.05),respectively,in the rat serum.The two extracts were also found to reduce the cholesterol content(-19.31％,P＜0.05;-23.73％,P＜0.05)and triglyceride content(-9.82％,P=0.11;-24.83％,P＜0.05)in rat liver.SEMD was also found to significantly reduce the total cholesterol(TC)level in rat serum(-13.31％,P＜0.05).These findings indicate that both stalk extracts effectively regulated lipid metabolism in rats and have the potential to be used as functional food ingredients for the prevention of cardiovascular disease.