不同温度胁迫下4种鲜食葡萄花粉活性差异OA

This study used four grape varieties('Italia''Zhengyan Wuhe''Wuhe Cuibao'and'Queen Nina')as materials to investigate the effects of sucrose,boric acid,and calcium nitrate concentrations on pollen germination through orthogonal tests and in vitro culture,and to screen for the optimal culture medium for each variety.The results showed that pollen germination of'Queen Nina'was most sensitive to calcium nitrate,while the other three varieties were mainly influenced by sucrose concentration.The optimal in vitro germination media for each variety were:250 g·L-1 sucrose+0.06 g·L-1 boric acid+0.09 g·L-1 calcium nitrate for'Italy';150 g·L-1 sucrose+0.06 g·L-1 boric acid for'Zhengyan Wuhe';150 g·L-1 sucrose+0.06 g·L-1 boric acid+0.06 g·L-1 calcium nitrate for'Wuhe Cuibao';and 250 g·L-1 sucrose+0.06 g·L-1 boric acid for'Queen Nina'.Under these optimal media,the pollen germination rates were in the order:'Zhengyan Wuhe'(67.87%)＞'Wuhe Cuibao'(54.34%)＞'Queen Nina'(49.27%)＞'Italy'(40.39%).Temperature experiments(15-36℃)indicated that both pollen germination rate and pollen tube length initially increased and then decreased with rising temperature,with the optimal temperature range being 24-30℃.Analysis using the Cumulative Stress Response Index(CSRI)classified'Italy'as an intermediate type,'Zhengyan Wuhe'as low-temperature sensitive and high-temperature tolerant,while'Wuhe Cuibao'and'Queen Nina'were identified as low-temperature tolerant and high-temperature sensitive.