不同发病程度'木纳格'葡萄酸腐病微生物群落差异研究OA

To reveal the variation patterns of microbial communities in'Munage'grapes under different degrees of sour rot disease severity,this study employed high-throughput sequencing technology to analyze bacterial and fungal communities in healthy and mildly,moderately,and severely infected fruits.Results showed that microbial α-diversity on fruit surfaces significantly decreased with increasing disease severity.In bacterial communities,the abundance of Proteobacteria and Firmicutes increased,Lactobacillus showed exponential growth,and Acetobacter became the dominant bacterial group.Fungal community diversity also significantly decreased,with Hanseniaspora abundance gradually declining as disease severity increased,while Saccharomycodes and Starmerella abundance increased significantly.LEfSe analysis identified characteristic biomarkers at each disease stage:for bacteria,Pseudomonas was identified in healthy fruits,Acetobacter group in mildly infected fruits,Fructobacillus in moderately infected fruits,and Lactobacillus in severely infected fruits;for fungi,Pichia was identified in healthy fruits,Hanseniaspora in mildly infected fruits,Hanseniaspora uvarum and Kazachstania in moderately infected fruits,and Saccharomycodes in severely infected fruits.This study systematically revealed the differences in microbial communities of'Munage'grape sour rot disease under different disease severities,providing a scientific basis for constructing precision prevention and control systems based on microbial ecological regulation.