黄水肽的分离鉴定及其潜在的血管紧张素转换酶抑制活性OA

Huangshui is a by-product generated during the Baijiu brewing process,and its resource utilization rate remains relatively low.In this study,high-value-added bioactive peptides were isolated and identified from Huangshui,and their potential angiotensin-con-verting enzyme(ACE)inhibitory activities were investigated to explore a novel approach for the resource utilization of Huangshui.A pep-tide fraction M3(molecular weight 0.15-1 kDa),characterized by high peptide content and ACE inhibitory activity,was prepared through organic solvent precipitation and membrane filtration.Subsequently,the M3 components were separated using macroporous resin column chromatography,and 61 peptides were identified via high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC/QTOF).The virtual screening of ACE inhibitory peptides using molecular docking revealed that the fol-lowing peptides exhibited promising inhibitory activity:Val-Tyr,Cyclo(Arg-Pro),Cyclo(Glu-Leu),Cyclo(Pro-Leu),Cyclo(Pro-Tyr),Cyclo(Leu-Arg),Pro-Val-Ala,and Pro-Val-Val.The binding energies between these eight peptides with the ACE target enzyme were all lower than-7 kcal/mol.These peptides can form hydrogen bonds and hydrophobic interactions with the residues in the ACE active cen-ter,thereby stabilizing the complex formation.This indicates their potential as effective ACE inhibitory peptides.This study successfully i-solated and identified 61 small peptides from Huangshui for the first time and screened out potential high-activity ACE inhibitory peptides through molecular docking.These findings provide valuable technical references for the development and utilization of Huangshui peptides,thereby promoting energy conservation and emission reduction in Baijiu production enterprises.