景芝镇高温大曲微生物群落与理化性质和感官品质的相关性分析OA

This study employed PacBio SMRT sequencing and pure culture technology to analyze the microbial community composi-tion of high-temperature Daqu.Conventional physicochemical analyses and bioassays were conducted to assess its physicochemical proper-ties and sensory qualities,as well as to investigate correlations with the dominant microorganisms.The results of the PacBio SMRT sequen-cing indicated that the bacterial community in high-temperature Daqu from Jingzhi Town exhibited significantly greater species richness and diversity compared to the fungal community(P＜0.05).Species-level analysis revealed that Kroppenstedtia sanguinis was the predominant bacterial species,with an average relative abundance of 35.60％.The main fungal species identified were Thermomyces lanuginosus,Ther-moascus crustaceus,and Thermoascus aurantiacus,with average relative abundances of 47.97％,16.81％,and 15.59％,respectively.Pure culture results indicated that the culturable microorganisms were primarily represented by Bacillus licheniformis and Bacillus amyloliq-uefaciens.Correlation analysis suggested that the bacterial taxa may have a more significant influence on the physicochemical properties and sensory quality of high-temperature Daqu from Jingzhi Town.This study enhances the understanding of the microbial community structure associated with high-temperature Daqu and provides valuable microbial strains that could support improvements in the Daqu production process and its quality.