黄芪多糖对嗜酸乳杆菌CICC 22162冷冻干燥保护效果的研究OA

Astragalus polysaccharide(APS)exhibits multiple physiological activities,making its efficient development and utilization highly significant.This study investigated the cryoprotective effects of purified APS(composed of 64.38％glucose,19.81％galactose,and 15.81％arabinose)in combination with other cryoprotectants on Lactobacillus acidophilus CICC 22162 during freeze-drying.Results showed that the APS-based cryoprotectant significantly improved bacterial survival to(85.02±1.25)％.Scanning electron microscopy re-vealed more uniform cellular morphology,compared with conventional cryoprotectants,the APS-based formulation also resulted in a 15.5％lower than conventional protectants,lactate dehydrogenase leakage decreased by 38.7％,viability reduction in lysozyme sensitivi-ty tests was 20.6％lower,NaCl-induced membrane damage was reduced by 37.2％,and histone phosphorylation leakage decreased by 68％.These findings proved APS better maintains cell wall/membrane integrity,reduced intracellular content leakage,and enhanced re-sistance to environmental stresses.The formulation achieved 81.3％survival in simulated gastrointestinal digestion(33.9％improvement over conventional protectants)and maintained 9.18 lg CFU/mL viability after 120 days at4℃.The APS-based cryoprotectant enables production of freeze-dried probiotics with high viability,excellent gastrointestinal tolerance,and superior stability,representing an innova-tive protection strategy with significant application value in functional foods and gut microbiota regulation.