联合宏基因组学及代谢组学探讨长双歧杆菌长亚种HSBL001后生元缓解结肠炎作用机制OA

Postbiotics are inactivated microorganisms or included their metabolites that benefit host health.Compared with probiotics,they have higher safety,stronger stability,and a broader range of action targets,thus showing significant advantages in alleviating inflam-matory bowel disease.This study evaluated the ameliorative effect of the postbiotic from Bifidobacterium longum subsp.longum HSBLOO1 on dextran sulfate sodium-induced ulcerative colitis mouse model,and explored the potential mechanism of this effect via metagenomics and metabolomics.Results indicate that HSBL001 significantly alleviates colitis-related symptoms,including reducing the disease activity index,increasing colon length,improving histopathological damage,regulating inflammatory cytokine secretion(increasing IL-10 while decreasing IL-6 and TNF-α),and alleviating splenomegaly.Metagenomic analysis showed that HSBL001 significantly increased the abun-dance of beneficial bacteria such as Duncaniella dubosii,Faecalibacterium rodentium,and Bacteroides thetaiotaomicron,while reducing the abundance of bacteria like Mucispirillum schaedleri and Pumilibacter muris.Metabolomic analysis revealed that HSBLO01 significant-ly upregulated 95 metabolites compared to the model group,including S-nitrosoglutathione,α-linolenic acid,and 7α-hydroxy-3-oxochol-est-4-en-27-oic acid,while significantly downregulating 80 metabolites including 3-hydroxyisovaleric acid and N-Acetyl-D-glucosaminitol.Pathway enrichment analysis of these differential metabolites shows that the postbiotic alleviates colitis mainly by regulating pathways such as primary bile acid biosynthesis and α-linolenic acid metabolism.In conclusion,supplementation with the HSBL001 effectively alleviates ulcerative colitis symptoms in mice.Its potential mechanisms are closely linked to regulating gut microbiota composition,increasing the a-bundance of bacteria such as D.dubosii and F.rodentium,as well as modulating primary bile acid biosynthesis and α-linolenic acid metab-olism.This study provides a new intervention method and research insights for alleviating colitis,and a theoretical basis for the industrial application of postbiotics.