遮阴处理对不同采收期马瑟兰葡萄和葡萄酒花色苷类物质的影响OA

In order to investigate the effects of different shading treatments on anthocyanins in grapes and wines,using Marselan grapes as the test ma-terial,the upper end of the tree was shaded with 20％and 50％shading nets(numbered as Q1 and Q2)at 5％veraison-complete maturity,and using the normal growth grape fruit without shading treatment as the control(CK).The contents of anthocyanins in grapes and wines at different harvest periods were detected by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Combined with orthogonal partial least squares-discriminant analysis(OPLS-DA),the differences in anthocyanin accumulation and composition of grape and wine under different shading treatments were analyzed.The results showed that shading treatment could effectively reduce the soluble solids and reducing sugar content of Marse-lan grapes,increase the total acid content,and had no significant effect on 100-grain weight and pH value.The quality of wine treated with Q1 was optimal,and the contents of total phenols,total tannins and total anthocyanins were the highest,which were 4 156.00 mg/L,3 929.80 mg/L and 3 156.00 mg/L,respectively.The content of methylated anthocyanins in wine with Q1 treatment(201.212 mg/L)was significantly higher than that of Q2 treatment(139.988 mg/L)and CK group(137.144 mg/L)(P＜0.05).The content of unacylated and acetylated anthocyanins in Q1 treated wine was 216.482 mg/L and 29.219 mg/L,respectively,which were significantly higher than Q2 treatment and CK group(P＜0.05),and also increased the main anthocyanin contents in grape peel and wine.Grapes and wines from CK,and Q1,Q2 treatments could be effectively distinguished by orthogonal par-tial least squares-discriminant analysis(OPLS-DA).