酶法结合化学改性对油棕粕膳食纤维理化性质与降血糖活性的影响OA
Effects of Enzymolysis Combined with Chemical Modification on Physicochemical Properties and Hypoglycemic Activity of Oil Palm Kernel Expeller Dietary Fiber
本文探究酶法结合羧甲基化或乙酰化改性对油棕粕膳食纤维的化学组成、结构、理化性质与降血糖活性的影响.结果表明,两种复合改性均使油棕粕膳食纤维的微观结构变得疏松多孔;FT-IR 光谱证实化学改性成功引入了羧甲基和乙酰基,且 3400 cm-1 附近吸收峰的伸缩振动说明复合改性破坏了纤维中的氢键.经酶法结合羧甲基改性和酶法结合乙酰化改性处理后,油棕粕纤维的可溶性膳食纤维含量从 4.00%显著增至 16.14%和 12.85%(P<0.05);持水力从2.06 g/g分别增至5.72 g/g和4.15 g/g;膨胀力从1.01 mL/g分别增至3.58 mL/g和2.46 mL/g;黏度从 4.08 cP分别增至 13.90 cP和 7.71 cP;葡萄糖吸附能力从 1.84 μmol/g分别增至 3.99 μmol/g和 4.53 μmol/g;葡萄糖扩散抑制能力从 16.32%分别增至 38.18%和 25.15%.此外,经酶法结合羧甲基处理的纤维,其α-淀粉酶抑制活性(19.41%)较未改性纤维提升了 0.43倍,而酶法结合乙酰化处理的纤维该活性并未得到提高.综上所述,酶法结合羧甲基或乙酰化均可一定程度增强油棕粕膳食纤维的理化性质与降血糖活性,其中酶法结合羧甲基改性的效果最优,这有助于扩大其在食品工业生产中的应用.
This study investigated the effects of enzymolysis combined with carboxymethylation or acetylation modification on the chemical composition,structure,physicochemical properties,and hypoglycemic activity of oil palm kernel expeller dietary fiber.The results demonstrated that both composite modification methods rendered the microstructure of oil palm kernel expeller dietary fiber more loose and porous.Fourier transform infrared(FT-IR)spectroscopy confirmed the successful introduction of carboxymethyl and acetyl groups via chemical modification,and the stretching vibration of the absorption peak around 3400 cm-1 indicated that the composite modifications disrupted the hydrogen bonds in the fiber.After enzymolysis combined with carboxymethylation and enzymolysis combined with acetylation,the soluble dietary fiber content of oil palm kernel expeller fiber increased significantly from 4.00%to 16.14%and 12.85%(P<0.05);water-holding capacity rose from 2.06 g/g to 5.72 g/g and 4.15 g/g;water-swelling capacity increased from 1.01 mL/g to 3.58 mL/g and 2.46 mL/g;viscosity increased from 4.08 cP to 13.90 cP and 7.71 cP;glucose adsorption capacity enhanced from 1.84 μmol/g to 3.99 μmol/g and 4.53 μmol/g;and glucose diffusion inhibition capacity improved from 16.32%to 38.18%and 25.15%,respectively.In addition,for the oil palm kernel expeller dietary fiber treated with enzymolysis combined with carboxymethylation,its α-amylase inhibitory activity(19.41%)was increased by 0.43 times compared with that of the unmodified fiber,while the activity of the oil palm kernel expeller dietary fiber treated with enzymolysis combined with acetylation was not improved.In conclusion,enzymolysis combined with either carboxymethylation or acetylation could enhance the physicochemical properties and hypoglycemic activity of oil palm kernel expeller dietary fiber to a certain extent,with enzymolysis combined with carboxymethylation yielding the best results.This thus expands its application potential in the food industry.
王楠;宋昕凌;马彪;刘心语;张怡萱;郑亚军;王浩
山西师范大学食品科学学院,山西 太原 030092山西师范大学食品科学学院,山西 太原 030092山西师范大学食品科学学院,山西 太原 030092山西师范大学食品科学学院,山西 太原 030092山西师范大学食品科学学院,山西 太原 030092山西师范大学食品科学学院,山西 太原 030092山西师范大学食品科学学院,山西 太原 030092
轻工纺织
油棕粕膳食纤维酶法改性羧甲基化乙酰化理化性质降血糖活性
oil palm kernel expeller dietary fiberenzymatic modificationcarboxymethylationacetylationphysicochemical propertieshypoglycemic activity
《食品工业科技》 2026 (5)
43-50,8
山西省自然科学基金面上项目(202203021221139)山西师范大学2025年大学生创新创业计划项目(2025DCXM-63)山西师范大学2025年大学生创新创业计划项目(2025DCXM-71).
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