减肥食品中酚丁类化合物筛查与质谱裂解规律OA

[Objective]To establish a screening method for illegally added oxyphenisatine and its derivatives in dietetic foods using ultra-performance liquid chromatography(UPLC)and UPLC quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).[Methods]A UV spectral library was constructed using reference standards,and a high-resolution mass spectral database containing retention time,precursor ions,and characteristic fragment ions was established.Tandem mass spectrometry(MS/MS)was employed to elucidate characteristic fragmentation pathways of oxyphenisatine compounds and to determine their diagnostic fragment ions.The UPLC-Q-TOF-MS method was further evaluated for data processing,specificity,limit of detection(LOD),and robustness.[Results]Oxyphenisatine compounds exhibited similar spectral features,with diagnostic fragment ions at m/z 224.070 6 and m/z 196.075 7.Chlorinated derivatives produced characteristic ions at m/z 258.032 1 and m/z 230.037 2,while fluorinated derivatives yielded ions at m/z 242.061 1 and m/z 214.066 2.The LODs ranged from 0.5 to 5.0 mg/kg.In actual sample analysis,one unknown oxyphenisatine compound was detected and confirmed to be a fluorinated diacetophenbutate.[Conclusion]This method demonstrates strong anti-interference capacity,good stability,and is suitable for the rapid screening and confirmatory analysis of oxyphenisatine compounds in dietetic foods.