外源H2O2对'无核白'葡萄ROS积累的影响OA

To investigate the distribution and response mechanisms of exogenous hydrogen peroxide(H2O2)on reactive oxygen species(ROS)accumulation pattern in grapes,tissue culture seedlings and potted seedlings of'Thompson Seedless'were treated with root incubation(0-6 mmol·L-1,7 d)and foliar spraying(0-800 mmol·L-1)H2O2 applications.ROS accumulation was visualized using nitrotetrazolium blue chloride(NBT)and diaminobenzidine(DAB)staining,while activities of catalase(CAT),superoxide dismutase(SOD),and glutathione reductase(GR)were determined.Results demonstrated that H2O2 were significantly accumulated in leaf margins but not around leaf veins when roots were treated with 2 mmol·L-1 H2O2.Leaf SOD activity peaked at the 2 mmol·L-1 treatment.Leaf CAT,GR and SOD activities were all significantly enhanced in 100 mmol·L-1 pot seedling treatment group.These findings indicate that different exogenous H2O2 applications affect ROS accumulation patterns in leaves.This study provided insights and knowledge for grape stress response mechanisms.