桑椹药渣粗多糖复合酶法提取工艺及抗氧化活性研究OA

An ultrasoundassisted complex enzyme extraction process was optimized to extract residual polysaccharides from Morus alba L.(mulberry)medicinal residue,and the in vitro antioxidant activity of the obtained crude polysaccharides was investigated to support comprehensive utilization of the residue.Mulberry residue was used as the raw material,single-factor experiments were initially conducted to select the optimal enzyme combination of cellulase and pectinase,the parameters including enzymolysis time,solid-to-liquid ratio(g/mL),enzyme concentration,pH and reaction temperature were optimized.An orthogonal experimental design was then applied to determine the optimal extraction conditions.Polysaccharide content was determined by the phenol-sulfuric acid method,and antioxidant activity was evaluated by DPPH and ABTS free radical scavenging assays.The optimal conditions identified by the orthogonal test were pH 4,enzymolysis temperature 40℃and complex enzyme concentration 1%,under which a polysaccharide yield of 443.15 mg/g was achieved.The in vitro antioxidant assays demonstrated that the mulberry polysaccharides exhibited significant radical scavenging capacity.The maximum scavenging rates were 49.37%(at 10 mg/mL)for DPPH free radicals and 79.39%(at 5 mg/mL)for ABTS free radicals,and the scavenging capacity was observed increasing with polysaccharide concentration.It was concluded that the ultrasound-assisted complex enzyme method was an efficient and effective process for extracting polysaccharides from mulberry residue.The extracted polysaccharides exhibited significant antioxidant activity,providing a theoretical foundation for the comprehensive utilization of mulberry resources and high-value development of this traditional Chinese medicine waste.