复合酶辅助提取瓶尔小草多糖工艺优化及其体外抗氧化活性OA

The enzymatic extraction of polysaccharides from Ophioglossum vulgatum was optimized,and the extract's in vitro antioxidant activity was evaluated.Based on the previous single factor tests,the response surface methodology(RSM)was applied.The independent variables were the liquid-to-solid ratio,extraction temperature,extraction time,and the concentration of the compound enzyme(cellulase∶papain at a mass ratio of 1∶1).Using polysaccharide extraction yield as the evaluation criterion,a four-factor,three-level response surface method was employed to optimize the extraction process for the polysaccharides from Ophioglossum vulgatum.The optimized enzymatic extraction process was determined as a liquid-to-solid ratio of 66∶1(mL/g),extraction temperature of 52℃,composite enzyme dosage of 0.94%and extraction time of 90 min.Under the above conditions,the yield of the polysaccharide was 13.84%,consistent with the predicted value.Furthermore,the extracted polysaccharide demonstrated good antioxidant potential,its scavenging ability against DPPH free radicals and ABTS free radicals were strong.The IC50 values were 40.25 and 48.07 μg/mL,respectively.These findings highlight that the polysaccharides from Ophioglossum vulgatum are a promising source of natural antioxidants.