响应面法优化香菇多糖提取工艺及抗氧化活性研究OA

Taking the residue of Lentinula edodes after polyphenol extraction as the raw material,polysaccharides were extracted by ultrasonic-assisted water extraction method.Using the lentinan yield as the evaluation index,the extraction process of lentinan was optimized by single-factor experiments combined with response surface methodology and the radical scavenging ability of lentinan was investigated.An in vivo antioxidant test model was constructed using Caenorhabditis elegans to investigate the effect of lentinan on oxidative stress-related indicators in C.elegans.The results showed that the optimal extraction conditions for L.edodes polysaccharides were as follows:ultrasonic power 240 W,solid-to-liquid ratio 1∶30(g∶ml),ultra-sonic time 50 min,and temperature 75 ℃.Under these optimal conditions,the yield of lentinan was 1.89％.In vitro antioxidant experiments results demonstrated that lentinan exhibited strong scavenging abilities against 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and hydroxyl radicals,with half-scavenging concentrations(IC50 value)of 1.91 mg/ml,0.58 mg/ml,and 0.16 mg/ml,respec-tively.In vivo antioxidant experimental results showed that lentinan significantly enhanced the activities of superoxide dismutase(SOD)and catalase(CAT)(P＜0.05),and significantly inhibited the accumulation of malondialdehyde(MDA)(P＜0.05)in the body of C.elegans.Therefore,lentinan had significant antioxidant activity.