鲁西北运河产区中高温和高温大曲真菌群落结构和水分指标解析及其相关性OA

To investigate the quality differences between medium-high-and high-temperature Daqu in the Northwest Canal region of Shandong province,the fungal community structure,and moisture indicators(water content,water activity,bound water,immobile water,and free water content)were an-alyzed by PacBio SMRT sequencing technique,conventional physicochemical testing methods,and low field-nuclear magnetic resonance(LF-NMR)technology.Additionally,the correlation between dominant fungal species and moisture indicators was analyzed.Sequencing results revealed that As-pergillus proliferans was the dominant species in medium-high-temperature Daqu,with an average relative abundance of 55.08％,whereas Thermo-mucor lanuginosus predominated in high-temperature Daqu,with an average relative abundance of 34.66％.A total of 12 significantly differential sub-stances were identified by linear discriminant analysis effect size(LEfSe),which showed distinct differences in the two types of Daqu samples.Among them,A.proliferans and T.lanuginosus could serve as biomarkers for medium-high-temperature Daqu and high-temperature Daqu,respectively.Moisture indicators analysis results demonstrated that medium-high-temperature Daqu had significantly higher water content(11.00％),water activity(0.641),immobile water(5.04％),and free water(0.96％)(P＜0.05),whereas high-temperature Daqu exhibited significantly higher bound water con-tent(94.96％)(P＜0.05).Correlation analysis results showed that there was a significant or extremely significant correlation between the dominant fungal species and the moisture indicators(P＜0.05 or P＜0.01).These findings provided data support for elucidating the quality variations among different types of Daqu in Northwest Canal region of Shandong province.