宽柄芥通过调节肠道菌群缓解葡聚糖硫酸钠诱导小鼠溃疡性结肠炎OA

Leaf mustard(LM)is an important raw material for making fermented vegetables such as suancai.To evaluate the nutri-tional components and functional activity of LM,the contents of dietary fiber,total glucosinolates and organic acids were determined.A mouse model of ulcerative colitis was established with 2.5％(w/w)sodium dextran sulfate.It was observed that feed with 5％leaf mus-tard powder influenced the body weight change,DAI,antioxidant capacity,serum cytokines levels and histopathological changes.And the mechanism was analyzed by intestinal microbiota diversity.Results that LM possessed 9.73 μmol/g of total glucosinolates and 19.39％di-etary fiber,and the organic acids contained citric acid,malic acid,succinic acid,and acetic acid,among which acetic acid had the high-est content.Compared to the model group,LM intervention restored the body weight,splenic and hepatic organ indexes of mice to levels similar to those of control group.LM significantly decreased the DAI scores,increased the colon length,and alleviated the damage to colon tissue.Meanwhile,LM decreased the MDA content by about 67.96％and improved the total antioxidant capacity by 83.95％.The inter-vention also reduced the levels of TNF-α and IL-6,while increased the expression of tight junction proteins ZO-1,occluding-1 and claudin and protected the integrity of colon tissue structure.LM intervention decreased the abundance of Escherichia-Shigella and Enterobacteriace-ae which were positively correlated with the levels of pro-inflammatory cytokine,and increased the proportion of beneficial bacteria such as Akkermansia and Blautia.The results show that LM could significantly alleviate the symptoms of colitis by maintaining the integrity of the intestinal barrier and improving gut microbial dysbiosis.