一种功能性奶粉对去卵巢大鼠骨骼健康的影响OA

The present study aims to investigate the effects of a functional milk powder supplemented with Davallia trichomanoides Blume on bone health in ovariectomized rats and its potential mechanisms.Thirty SPF-grade female SD rats weighing approximately 300 g were randomly divided into a sham-operated group,an ovariectomized(OVX)control group and an OVX+milk powder group,and were gavaged for 90 d.Calcium metabolism experiments were performed on the last three days of the intervention period.At the end of the inter-vention,the routine indexes of femur,bone mineral density and bone calcium were detected;The bone microstructure of femur and lumbar spine was scanned by Micro-CT,and key proteins expressions of bone metabolism of lumbar spine were analyzed by immunohistochemis-try.In addition,the mRNA expression levels of Wnt3a,β-catenin,RUNX2,and LEF-1 in the signaling pathways related to the mainte-nance of bone homeostasis were detected in the right tibia.Results showed that compared with the sham-operated group,the OVX control rats showed a significant decrease in the bone mineral density of the distal femur(P＜0.01),and the midpoint bone mineral density and bone calcium levels were significantly reduced in the OVX control group(P＜0.05).Compared with the OVX control group,the OVX+milk powder group showed significantly higher bone mineral density and bone calcium levels at the distal and midpoint of the femur,as well as different degrees of improvement in bone microstructure(P＜0.05).Compared with the OVX control group,the OVX+milk pow-der group showed significantly higher levels of VEGF protein expression in the lumbar spine(P＜0.01),and significantly higher levels ofβ-catenin,RUNX2,and LEF-1 mRNA expression in the tibia(P＜0.05 or 0.01).This functional milk powder can promote the absorp-tion and utilization of calcium,increase bone mineral density and bone calcium content,and thus improve the bone health of ovariecto-mized rats,and its mechanism may relate to Wnt/β-catenin signaling pathway.