高效生物胺降解菌酿酒酵母Z5的降胺机制OA
Mechanism of Biogenic Amine Degradation by a Highly Efficient Biogenic Amine-Degrading Strain,Saccharomyces cerevisiae Z5
以具有生物胺降解功能的野生酿酒酵母Hl和人工选育的酿酒酵母Z5为研究对象,通过转录组和代谢组分析探索其生物胺降解机制和关键代谢途径.结果表明,FMS1基因编码的多胺氧化酶在生物胺降解中起到至关重要的作用,尤其是对于亚精胺和精胺的降解.人工选育的酿酒酵母Z5通过调节多胺的代谢途径提高了其在胺胁迫条件下的生物胺降解效率.此外,谷胱甘肽代谢途径也在人工选育酿酒酵母Z5的降胺过程中发挥重要作用.通过多胺氧化酶活性测定,人工选育的酿酒酵母Z5在生物胺胁迫下的多胺氧化酶活性显著高于野生酿酒酵母菌Hl(P＜0.05).
The mechanism of biogenic amine degradation by the Saccharomyces cerevisiae wild-type strain Hl and the mutant strain Z5 and related metabolic pathways were explored through transcriptomic and metabolomic analyses.It was found that polyamine oxidase encoded by the FMS1 gene played a crucial role in degrading biogenic amines,particularly spermidine and spermine.Under amine stress,the mutant strain Z5 enhanced its degradation efficiency for biogenic amines by regulating polyamine metabolic pathways.Additionally,the glutathione metabolism pathway was found to play an important role in the amine degradation process of strain Z5.Its polyamine oxidase activity under amine stress was significantly higher than that of the wild-type strain Hl(P<0.05).
樊蓉;陈力;王丽;谭旭;姜子怡;李珂;王远亮
湖南农业大学食品科学技术学院,湖南 长沙 410125湖南农业大学食品科学技术学院,湖南 长沙 410125湖南农业大学食品科学技术学院,湖南 长沙 410125湖南农业大学食品科学技术学院,湖南 长沙 410125湖南农业大学食品科学技术学院,湖南 长沙 410125湖南农业大学食品科学技术学院,湖南 长沙 410125湖南农业大学食品科学技术学院,湖南 长沙 410125
轻工纺织
生物胺酿酒酵母组学分析多胺氧化酶降解机制
biogenic aminesSaccharomyces cerevisiaeomics analysispolyamine oxidasedegradation mechanism
《肉类研究》 2026 (3)
17-25,9
国家自然科学基金青年科学基金项目(31000809)湖南省生猪产业技术体系加工岗位专家项目(HARS-05)
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