巴戟天提取物对慢性束缚抑郁小鼠肠道菌群的影响OA

Objective:To investigate the effects and mechanisms of Morinda officinalis How.on the gut microbiota of chronic restraint stress(CRS)depression model mice.Methods:Eight-week-old male Kunming mice were randomly divided into six groups based on their body weight:control,model,fluoxetine,and M.officinalis How.low-(25 mg/kg/d),medium-(50 mg/kg/d),and high-dose(100 mg/kg/d)groups were established.CRS was used to establish a mouse model of depression.Depression-like behavior was observed using the sucrose preference(SPT)and tail suspension tests(TST).Morphological changes in the liver,kidneys,and testes were observed using hematoxylin and eosin(HE)staining.Serum 5-hydroxytryptamine(5-HT)levels were measured using enzyme-linked immunosorbent assay(ELISA).Finally,the gut microbiota were analyzed using 16S rRNA gene amplicon sequencing.Results:Compared with the control group,the sucrose preference rate of mice in the model group was significantly reduced,tail suspension time was significantly prolonged(P<0.05),and serum 5-HT level was significantly increased(P<0.01).This caused varying degrees of damage in the liver,kidneys,and testicles,as well as gut microbiota imbalance.After the intervention,50 mg/kg/d M.officinalis How.showed good antidepressant effects.Its sucrose preference rate was significantly increased,and tail suspension time was significantly shortened(P<0.05).5-HT levels were significantly reduced(P<0.01),liver,kidney,and testicular damage returned to normal levels.The abundance of Proteobacteria was significantly increased(P<0.05),and the abundance of Oscillibacter,Eubacterium_xylanophilum_group,Kurthia and unidentified bacterium were increased(P<0.05).Conclusion:The 50 mg/kg/d M.officinalis How.regulates gut microbiota imbalance and improves depression-like behavior in CRS mice.