南瓜籽肽改善运动疲劳作用OA
Effect of Pumpkin Seed Peptide on Improving Sports Fatigue
本研究建立疲劳小鼠模型,探究不同剂量的南瓜籽肽对小鼠疲劳的改善作用.45只小鼠被随机分为 5组,分别是正常对照组、模型组、南瓜籽肽低、中和高剂量组.分析南瓜籽肽的氨基酸组成,并探究不同剂量南瓜籽肽干预后,小鼠力竭游泳时间、血清有害物质、能量物质、氧化应激、炎症水平的变化.结果表明,南瓜籽肽由Glu、Leu和Pro等 18种氨基酸组成.动物实验表明,与模型组相比,高剂量南瓜籽肽处理后,小鼠游泳时间、肌糖原和肝糖原含量显著增加(P<0.05),血清尿素氮、乳酸、乳酸脱氢酶含量显著降低(P<0.05).同时,除肝糖原和乳酸脱氢酶含量外,高剂量组对上述的其他指标改善效果显著(P<0.05)优于低剂量和中剂量组.此外,相比于模型组,高剂量南瓜籽肽喂养的小鼠血清和肝脏中抗氧化酶超氧化物歧化酶(SOD,Superoxide dismutase)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)和过氧化氢酶(Catalase,CAT)显著增加(P<0.05),血清和肝脏中丙二醛(Malondialdehyde,MDA)显著降低(P<0.05).对小鼠炎症水平分析发现,高剂量南瓜籽肽组促炎细胞因子白细胞介素-6(Interleukin 6,IL-6)、肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)和白细胞介素-1β(Interleukin-1β,IL-1β)显著低于(P<0.05)模型组,抗炎细胞因子白细胞介素-4(Interleukin-4,IL-4)和白细胞介素-10(Interleukin-10,IL-10)显著高于(P<0.05)模型组.最后,相关性分析表明Trp和Leu与促炎细胞因子和血清有害物质(乳酸脱氢酶和血清尿素氮)呈显著负相关(P<0.05).综上,南瓜籽肽具有抗疲劳作用,高剂量南瓜籽肽作用效果最佳.本研究为南瓜籽肽的应用提供理论支撑.
In this study,a fatigue mouse model was developed to investigate the effects of different doses of pumpkin seed peptide on fatigue in mice.Forty-five mice were randomly assigned to five groups:normal control group,model group,and three pumpkin seed peptide groups with low,medium,and high-doses.Firstly,the amino acid composition of the pumpkin seed peptide was analyzed.Secondly,the changes in swimming time,serum harmful substances,energy substances,oxidative stress,and inflammation levels in mice were examined following intervention with different doses of pumpkin seed peptide.The results revealed that the pumpkin seed peptide contained 18 amino acids,including Glu,Leu,and Pro.Animal experiments demonstrated that compared to the model group,swimming time,muscle glycogen,and hepatic glycogen levels were significantly increased(P<0.05),while serum urea nitrogen,lactic acid,and lactate dehydrogenase levels were significantly reduced(P<0.05)in mice treated with a high dose of pumpkin seed peptide.Additionally,the high-dose group exhibited significantly greater improvements(P<0.05)in the aforementioned indices compared to the low-and medium-dose groups,except for liver glycogen and lactate dehydrogenase levels.Furthermore,in the high-dose pumpkin seed peptide group,the antioxidant enzymes SOD,GSH-Px,and CAT were significantly elevated(P<0.05)in both serum and liver tissues,whereas MDA levels were significantly reduced(P<0.05).Analysis of inflammatory markers revealed that pro-inflammatory cytokines(IL-6,TNF-α,and IL-1β)were significantly reduced(P<0.05),while anti-inflammatory cytokines(IL-4 and IL-10)were significantly elevated(P<0.05)compared to the model group.Correlation analysis further revealed that Trp and Leu exhibited significant negative correlations(P<0.05)with pro-inflammatory cytokines and serum markers(lactate dehydrogenase and urea nitrogen).In conclusion,pumpkin seed peptide demonstrates anti-fatigue properties,with high-dose administration exerted the most pronounced effects.This study provides a theoretical foundation for the application of pumpkin seed peptide in fatigue management.
SHAN Yi;LIANG Weiwei;LIU Limei;OUYANG Le;BIAN Chun
School of Food Engineering,Harbin University,Harbin 150086,ChinaSchool of Food Engineering,Harbin University,Harbin 150086,ChinaSchool of Food Engineering,Harbin University,Harbin 150086,ChinaSchool of Food Engineering,Harbin University,Harbin 150086,ChinaSchool of Food Engineering,Harbin University,Harbin 150086,China
农业科技
南瓜籽肽抗疲劳氧化应激能量代谢炎症
pumpkin seed peptideanti-fatigueoxidative stressenergy metabolisminflammation
《食品工业科技》 2026 (2)
429-436,8
哈尔滨学院青年博士科研启动基金项目(HUDF2021113).
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