PEG引发和渗透处理对山梨种子热休眠的预防作用研究
Effects of PEG Initiation and Osmotic Treatment on Thermal dormancy of Pyrus ussuriensis seeds
[目的]为探究PEG引发和渗透处理对山梨(Pyrus ussuriensis)种子热休眠诱导的影响。[方法]以解除初生休眠的山梨种子为试验材料,研究不同质量分数(5%、25%、50%)聚乙二醇(PEG)溶液引发处理不同时间(0.5 d、1 d、2 d)和渗透处理不同时间(1 d、3 d、7 d)后,种子在高温(25℃)下的萌发表现。[结果]PEG 引发和渗透处理能够减弱山梨种子热休眠的诱导,在一定条件下达到预防的效果。采用质量分数25%的PEG溶液对山梨种子进行1 d的引发处理时,种子发芽率较对照组发芽率(7%)显著提高 57%,发芽指数显著高于对照 0.65,平均发芽时间显著缩短 7.88 d.采用质量分数为 25%PEG溶液对山梨种子渗透处理 1 d,种子的发芽率(47%)显著高于对照 40%,发芽指数显著高于对照 0.53。[结论]山梨种子在高温条件下,采用 25%PEG溶液对种子引发或渗透处理 1 d可有效阻止山梨种子进入热休眠。
[Objective]In order to investigate the effects of PEG initiation and osmotic treatment on the induction of thermodormancy of Pyrus ussuriensis seeds.[Methods]The primary dormancy removal of P.ussuriensis seeds was used as experimental materials.The germination performance of seeds under high temperature(25℃)was studied after different mass fractions(5%,25%,50%)of polyethylene glycol(PEG)solution were treated for different time(0.5 d,1 d,2 d)and osmotic treatment for different time(1 d,3 d,7 d).[Results]PEG initiation and osmotic treatment could weaken the induction of thermal dormancy of P.ussuriensis seeds and achieve the preventive effect under certain conditions.When the seed was treated with 25%PEG solution for 1 d,the germination percentage was significantly increased by 57%compared with the control(7%),the germination index was significantly higher than that of the control group(0.65),and the mean germination time was significantly shortened by 7.88 d.The seed germination percentage(47%)was significantly higher than that of the control(40%),and the germination index was significantly higher than that of the control(0.53).[Conclusion]Under the condition of high temperature,the seed initiation or osmotic treatment for 1 d with 25%PEG solution could effectively prevent the seed from inducing thermal dormancy.
董博文;刘婷;张鹏;
东北林业大学林学院,森林生态系统可持续经营教育部重点实验室,哈尔滨 150040;东北林业大学林学院,森林生态系统可持续经营教育部重点实验室,哈尔滨 150040;东北林业大学林学院,森林生态系统可持续经营教育部重点实验室,哈尔滨 150040;
Pyrus ussuriensis Thermal dormancy Initiation treatment Osmotic treatment Seed germination
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