超声波辅助提取刺五加总黄酮及其工艺优化＊
Determination of total flavonoids in Acanthopanax senticosus by enzymolysis and its technological optimization
目的:优化刺五加总黄酮超声提取工艺，对比分析黑龙江省四个地区的 21 种刺五加总黄酮含量情况。方法:通过Box-Behnken响应面方法对超声提取工艺进行优化，并对 21 种不同产地刺五加总黄酮含量进行聚类分析。结果:确定最优工艺条件是超声时间30min，料液比 1:12，超声功率250w，总黄酮得率平均为1.453mg/g.结论:实验通过优化超声辅助提取法，比较不同产地刺五加总黄酮含量，为今后提取工艺的优化提供一定的数据支持，对中药材的品质分析奠定一定的理论基础。
Objective: To optimize the ultrasonic extraction technology of total flavonoids from Acan thopanax and compare and analyze the content of total flavonoids from 21 kinds of Acanthopanax in four regions of Heilongjiang Province.Methods: The ultrasonic extraction technology was optimized by Box-Behnken response surface method, and the total flavonoids content of 21 kinds of Acanthopanax from different producing areas were analyzed by cluster analysis.Results: The optimal process conditions were determined as ultrasonic time 30min, solid-liquid ratio 1:12, ultrasonic power 250w, and the average yield of total flavonoids was 1.453mg/g.Conclusion: By optimizing the ultrasonic-assisted extraction method, the total flavonoid content of Acanthopanax from different producing areas was compared in the experiment, which provided certain data support for the optimization of the extraction process in the future and laid a certain theoretical foundation for the quality analysis of Chinese medicinal materials.
张顺捷;张丹;毕连东;仇有文;
;黑龙江省林业科学院牡丹江分院,黑龙江省 牡丹江 151700;黑龙江省林业科学院牡丹江分院,黑龙江省 牡丹江 151700;东北农业大学生命科学学院,黑龙江省 哈尔滨 150030;
R282.5
刺五加 总黄酮 Box-Behnken响应面试验 超声辅助提取法 聚类分析
Acanthopanax senticosus Total flavone Box-Behnken response surface test Ultrasonic-assisted extraction
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