Variations in methane emissions from dairy cows:associations with rumen microbial synergy and metabolic pathway divergenceOA
Background Methane(CH_(4))is a metabolic by-product of rumen microbial fermentation,contributing significantly to global warming and dietary energy loss.Elucidating the mechanisms underlying natural variation in rumen methanogenesis is essential for the development of effective CH_(4)mitigation strategies.Here,we applied rumen metagenomics to identify the microbial mechanisms for differences in enteric CH_(4)emissions among dairy cows.Results Enteric CH_(4)emissions from 111 lactating dairy cows under normal feeding conditions were utilized to characterize the natural variation in rumen methanogenesis.Metagenomic analysis revealed that the comprehensive effects of bacteria involved in starch degradation,lactate metabolism,and volatile fatty acid biosynthesis provide distinct amounts of hydrogen for rumen methanogenesis in high-methane-producing(HMP)and low-methane-producing(LMP)cows.Ciliate protozoa were universally abundant in HMP cows(P<0.05),whereas methanogens enrichment exhibited heterogeneity,with the dominant methanogen Methanobrevibacter exhibiting negative correlations with the other 11 methanogens(P<0.05).Six nutrient metabolic pathways modulating methanogenesis were identified,and HMP-associated methanogenesis was further driven by upregulated formate metabolism and acetoclastic pathways(P<0.05).Random forest model analysis screened 34 microbial genera as biomarkers for CH_(4)production.Conclusions This study excluded extrinsic confounders exist for rumen microbiome and CH_(4)emissions in dairy cows.These findings elucidated the causal microbial and metabolic mechanisms underlying rumen methanogenesis,providing actionable targets for microbiome-based strategies to mitigate CH_(4)emissions from livestock farming.
Peng Jia;Lifeng Dong;Tao Ma;Yanliang Bi;Yan Tu;Qiyu Diao
Institute of Feed Research,Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Beijing 100081,People’s Republic of China Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences/Key Laboratory of Livestock and Poultry Resources(Pig)Evaluation and Utilization of Ministry of Agriculture and Rural Affairs/Shanghai Engineering Research Center of Breeding Pig,Shanghai 201106,People’s Republic of ChinaInstitute of Feed Research,Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Beijing 100081,People’s Republic of ChinaInstitute of Feed Research,Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Beijing 100081,People’s Republic of ChinaInstitute of Feed Research,Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Beijing 100081,People’s Republic of ChinaInstitute of Feed Research,Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Beijing 100081,People’s Republic of ChinaInstitute of Feed Research,Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Beijing 100081,People’s Republic of China
农业科技
Dairy cowsMethaneMethane mitigationRumen methanogenesisRumen microbiota
《Journal of Animal Science and Biotechnology》 2026 (5)
P.2987-3005,19
funded by the National key Research and Development Program(2024YFD1300200)the Agricultural Science and Technology Innovation Program(CAAS-ASTIP).
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