重要病虫害抗药性机制与精准治理技术研究进展OA

Pesticide resistance has become a major scientific challenge constraining the green control of crop pests and diseases and the reduction and efficiency enhancement of pesticide use in China.Supported by the National Key Research and Development Program of China project"Research on Resistance Mechanisms and Precision Management Technologies for Major Pests and Pathogens",the research team conducted systematic studies on key areas including resistance formation mechanisms,resistance risk evolution patterns,molecular monitoring and early warning,and precision management of major crop pests and pathogens.These studies revealed common patterns underlying both target-site and non-target-site resistance in insect pests and plant pathogens and identified 25 key resistance genes.Six technologies for resistance gene prediction and functional validation were established,providing new technical routes for resistance research in non-model pests and pathogens.Based on key resistance genes and mutation sites,18 rapid diagnostic and early-warning technologies were developed,facilitating the transition of resistance monitoring from phenotype-based detection to genotype-based early warning.In addition,the resistance risks and adaptive evolutionary characteristics of several novel pesticides were systematically evaluated.Furthermore,eight precision resistance management technology systems were integrated,accompanied by the establishment of nine technical standards,with a cumulative demonstration and extension area of 383 000 hm2 in major agricultural production regions.This review systematically summarizes the above research advances,with the aim of providing references for resistance monitoring,early warning,and sustainable management of crop pests and pathogens in China.