水稻PDR基因家族鉴定及盐胁迫下的表达分析OA

The pleiotropic drug resistance(PDR)proteins,which belong to the ABCG subfamily in the ATP-binding cassette(ABC)transporter protein superfamily,play crucial roles in plant stress responses.To investigate the responses of the PDR gene family in rice to salt stress,this study identified members of this family in rice,and conducted bioinformatics analyses of their physicochemical properties,evolutionary relationships,and structural characteristics,etc.Additionally,the expression responses of OsPDR genes to salt stress were examined.The results revealed that a total of 16 PDR genes were identified in rice.Phylogenetic analysis results showed that OsPDR genes were more closely related to the homologous genes in maize than to Arabidopsis thaliana.OsPDR genes were classified into five groups(Group Ⅰ～Ⅴ),with members in the same group exhibiting similar gene structures and being highly conserved.A total of 35 cis-acting elements associated with the light response,hormone response,growth and development response,and adversity stress response were identified in the promoter regions of these genes.Transcriptomic data analysis results indicated that OsPDR genes were expressed in various tissues of rice,with relatively higher expression levels observed in roots for most OsPDR genes.Furthermore,the relative expression levels of 16 OsPDR genes were examined at the seedling stage of rice under normal and salt stress conditions.The results demonstrated that OsPDR genes were significantly regulated by salt stress,and different OsPDR genes might play key roles at different stages of the salt stress response.This study systematically identified members of the PDR gene family in rice,providing preliminary insights into their potential functions in salt stress responses,and offering references for the mining of salt-tolerant genes of rice and the breeding of salt-tolerant rice varieties.