云南昭通老龄苹果园不同坡段土壤细菌群落特征OA

This study focused on soils collected from different sampling sites within a 25-year-old apple orchard located in Zhaotong City,Yunnan Province,aiming to characterize the bacterial community structure and diversity across different collection points in a sloping aged apple orchard of this region.High-throughput sequencing on the Illumina platform was employed to analyze bacterial diversity in four soil groups:upper-slope soil,mid-slope soil,lower-slope soil,and continuous cropping soil.The results showed that the order of soil bacterial community richness among the different collection sites was:continuous cropping soil＞lower-slope soil＞mid-slope soil＞upper-slope soil,while the order of diversity was:continuous cropping soil＞mid-slope soil＞lower-slope soil＞upper-slope soil.At the phylum level,Actinobacteriota,Proteobacteria,Chloroflexi,and Acidobacteriota were the dominant phyla,with their combined relative abundances ranging from 81.20％to 84.63％.At the genus level,significant differences were observed among the different collection sites;the beneficial genera Bradyrhizobium and Gemmatimonas were notably enriched in the mid-slope soil.LEfSe analysis identified 88 differential taxa across the the different collection sites.The most influential differential taxa were Chloroflexi in the continuous cropping soil,Proteobacteria in the mid-slope and upper-slope soils,and Actinobacteriota in the lower-slope soil.Among the four collection sites,the mid-slope soil harbored a greater abundance of beneficial genera.