基于高效液相色谱-蒸发光散射检测法测定黄精中果糖含量OACHSSCD

This article establishes a method for determining the fructose content in Polygonati rhizoma medicinal materials using High Performance Liquid Chromatography-Evaporative Light Scattering Detection(HPLC-ELSD).The results showed that fructose had a good linear relationship within the linear range(R2=0.998 8),with precision and repeatability meeting the requirements,and an average recovery rate of 97.10%.The fructose content range of 15 batches of commercially available Polygonati rhizoma medicinal materials is 24.92 mg/g to 39.18 mg/g,with an average content of 31.63 mg/g.This method has the advantages of simple pre-processing,short operation cycle,accurate and reliable results,and can be used as a supplementary method for evaluating the quality of Polygonati rhizoma.