外源性抗氧化剂对哺乳动物卵母细胞冷冻保存的作用OA

Oocyte cryopreservation holds significant value in biotechnological applications including germplasm resource preservation,in vitro embryo production,and somatic cell cloning.However,cryopreservation-induced oxidative stress impairs the developmental potential of mammalian oocytes.While endogenous antioxidant enzymes in oocytes cannot completely eliminate excessive reactive oxygen species(ROS),supplementation of exogenous antioxidants in cryopreservation media have been employed to reduce oxidative damage.This review comprehensively examines the various forms of oxidative stress induced by cryopreservation in mammalian oocytes,with particular focus on elucidating the regulatory mechanisms of both non-enzymatic and enzymatic antioxidant defense systems.Moreover,we critically evaluate the efficacy and underlying mechanisms of diverse antioxidants in counteracting oxidative damage,thereby providing valuable references for enhancing the efficiency of mammalian oocyte cryopreservation.