基于血清代谢组学对羔羊早期生长发育速度的差异分析OA

[Objective]This study aimed to elucidate the intrinsic mechanisms underlying the differences in average daily gain(ADG)among Xinggao mutton lambs from a metabolomic perspective.Specifically,this study sought to screen for key metabolites and pathways influencing lamb growth performance,thereby providing a scientific basis for improving productivity.[Method]This study used 321 Xinggao mutton lambs as the experimental population.Body weight was measured within 48 hours of birth,and weaning weight was recorded at 50-60 days of age to calculate the average daily gain(ADG).Sixty male lambs were randomly divided into two groups of 30 each:the high average daily gain group(HADG)and the low average daily gain group(LADG).Blood samples were collected on the day of weaning to isolate serum,and serum metabolomic profiles were analyzed using untargeted metabolomics techniques to identify potential causes of differences in growth performance.[Result]Metabolomic analysis revealed a total of 108 significantly differentially expressed metabolites in the serum of lambs between HADG and LADG groups(P<0.05).Compared with LADG group,the serum of lambs in HADG group showed 77 significantly upregulated metabolites(primarily unsaturated fatty acids,long-chain acylcarnitines,amino acids,and their derivatives)and 31 significantly downregulated metabolites(primarily short-chain acylcarnitines).The differentially expressed metabolites were primarily enriched in pathways such as unsaturated fatty acid biosynthesis,tryptophan metabolism,and steroid hormone biosynthesis.After multiple rounds of screening,27 key metabolites were identified,among which 11-deoxy-PGE2,7-hydroxyolanzapine,tetraethylene glycol,1-phenyl-1-propanol,and carmustine-exhibited excellent discriminatory performance(AUC>0.9).Strong correlations were observed among the key metabolites(|r|>0.5).Specifically,11-deoxy-PG E2 showed a significantly strong positive correlation with ginkgolic acid(r=0.91,P<0.05),and 3-hydroxybutyrylcarnitine showed a significant positive correlation with isobutyrylcarnitine(C4)(r=0.81,P<0.05),and 3-hydroxybutyrylcarnitine was significantly negatively correlated with nicotinamide(r=-0.54,P<0.05).[Conclusion]In summary,lipid metabolism(unsaturated fatty acid synthesis and acylcarnitine regulation)and amino acid metabolism(branched-chain amino acids and tryptophan metabolism)were the core metabolic pathways underlying differences in average daily gain among Xinggao mutton lambs,the 27 potential biomarkers including 11-deoxy-PGE2,7-hydroxyolanzapine,tetraethylene glycol,1-phenyl-1-propanol,identified could provide important references for the precise regulation of lamb growth performance and related research.