妇炎清颗粒通过Notch信号通路调节Th17/Treg免疫平衡治疗盆腔炎性疾病的机制研究OA
Therapeutic mechanism of Fuyanqing granules in pelvic inflammatory disease by regulating Th17/Treg immune balance via the Notch signaling pathway
目的 探讨妇炎清颗粒治疗盆腔炎性疾病的作用机制.方法 60 只 SD 大鼠按照随机数字表法随机选取 15 只作为正常组,其余 45 只作为造模组,采用机械搔刮+脂多糖法制备盆腔炎性疾病大鼠模型.造模成功后,正常组不做干预,造模组随机分为模型组、妇炎清颗粒低、中、高剂量组(2、4、8 g/(kg·d))、妇科千金胶囊组(0.52 g/(kg·d)),连续灌胃给药 28 d.HE 染色观察大鼠子宫组织病理形态变化,ELISA 法检测大鼠血清 IgG、IgA、IgM、sICAM-1、TNF-α、IL-1β 的浓度,RT-qPCR 法检测大鼠子宫组织 RORγT、IL-17、Foxp3、IL-10 的 mRNA 表达,IHC 法检测大鼠子宫组织 Notch1、Notch2、Jagged1、Jagged2、Hes1、Hes5、ICAM-1 的阳性表达,Western blot 法检测大鼠子宫组织 Notch1、Notch2、Jagged1、Jagged2、Hes1、Hes5、RORγT、IL-17、Foxp3、IL-10的蛋白表达.结果 妇炎清颗粒能够减轻盆腔炎性疾病大鼠子宫组织充血、水肿、黏连及炎性浸润,提高血清IgG、IgA、IgM 浓度(P<0.01),降低 TNF-α、IL-1β、sICAM-1浓度(P<0.05,P<0.01),上调子宫组织 Foxp3、IL-10表达水平(P<0.05,P<0.01),下调 RORγT、IL-17、ICAM-1 表达水平(P<0.05,P<0.01),恢复 Th17/Treg 免疫平衡,抑制 Notch 信号通路配体(Jagged1、Jagged2)、受体(Notch1、Notch2)及效应基因(Hes1、Hes5)的过度激活,与妇科千金胶囊治疗作用相当.结论 妇炎清颗粒可能通过抑制 Notch 信号通路,调节 Th17/Treg 免疫平衡,增强免疫功能,改善炎症状态,治疗盆腔炎性疾病.
Objective To investigate the therapeutic mechanism of Fuyanqing granules in pelvic inflammatory disease(PID).Methods Sixty Sprague-Dawley rats were randomly assigned to the Control group(n=15)and modeling group(n=45).PID was induced by mechanical scratching combined with lipopolysaccharide administration.Following successful model establishment,rats were randomly assigned to Model group(equal volume of normal saline),FYQ-L,FYQ-M,FYQ-H group(2,4,8 g/(kg·d),respectively),and FKQJ group(0.52 g/(kg·d)).Treatment groups received intragastric administration for 28 consecutive days.Uterine histopathology was evaluated by hematoxylin and eosin staining.Serum concentrations of immunoglobulin(Ig)G,IgA,IgM,soluble intercellular adhesion molecule-1(sICAM-1),tumor necrosis factor-α(TNF-α),and interleukin(IL)-1β were measured by enzyme-linked immunosorbent assays.RT-qPCR and immunohistochemistry were used to examine the mRNA expression of RORγT,IL-17,Foxp3,and IL-10,and the protein expression of Notch1,Notch2,Jagged1,Jagged2,Hes1,Hes5,and ICAM-1,respectively,in uterine tissues.Western blot was used to quantify Notch1,Notch2,Jagged1,Jagged2,Hes1,Hes5,RORγT,IL-17,Foxp3,and IL-10 in uterine tissues.Results Fuyanqing granules alleviated uterine congestion,edema,adhesion,and inflammatory infiltration in PID rats.Treatment increased the serum concentrations of IgG,IgA,and IgM(P<0.01)while reducing the levels of TNF-α,IL-1β,and sICAM-1(P<0.05,P<0.01).In uterine tissues,it upregulated the expression of Foxp3 and IL-10(P<0.05,P<0.01),and downregulated the expression of RORγT,IL-17,and ICAM-1(P<0.05,P<0.01),indicating restoration of the helper T cell 17(Th17)/regulatory T cell(Treg)immune balance.Fuyanqing granules also inhibited the overexpression of Notch signaling pathway ligands(Jagged1,Jagged2),receptors(Notch1,Notch2),and target genes(Hes1,Hes5).These therapeutic effects were comparable to those of Fuke Qianjin capsules.Conclusions Fuyanqing granules may ameliorate PID by inhibiting the Notch signaling pathway,restoring the Th17/Treg immune balance,enhancing immune function,and improving the inflammatory microenvironment.
孙婷;杨欢欢;黄缨
湖北中医药大学附属荆州市中医医院,湖北 荆州 434000湖北中医药大学中医学院,武汉 430000湖北中医药大学附属荆州市中医医院,湖北 荆州 434000
医药卫生
妇炎清颗粒盆腔炎性疾病Notch信号通路Th17/Treg免疫平衡
Fuyanqing granulespelvic inflammatory diseaseNotch signaling pathwayTh17/Treg immune balance
《中国比较医学杂志》 2026 (18)
24-35,12
湖北省自然科学基金一般面上项目(2024AFB983)湖北省医学领军人才培养工程项目(鄂卫通[2019]47号)荆州市中医医院妇科科研创新团队项目(荆中医发[2025]37号).
评论