艾灸及艾烟调控p38 MAPK/NF-κB信号通路改善哮喘大鼠气道炎症的机制研究OA
The effect of moxibustion and moxa smoke on the airway inflammation in the asthmatic rats by regulating the p38 MAPK/NF-κB signaling pathway
目的 基于p38 MAPK/NF-κB 信号通路,探讨艾灸及艾烟改善哮喘大鼠气道炎症的作用机制.方法 SD 雄性大鼠随机分为正常组、模型组、地塞米松组、艾灸组、无烟艾灸组、艾烟熏吸组和香烟熏吸组,采用卵清白蛋白(OVA)致敏和激发建立哮喘大鼠模型,造模完成后,地塞米松组腹腔注射地塞米松磷酸钠溶液,艾灸组艾灸大鼠双侧"肺俞"、单侧"大椎"、双侧"风门"30 min,无烟艾灸组治疗方法及疗程同艾灸组,以上干预均每天 1 次,连续 14 d,模型组、正常组大鼠不做任何干预.观察大鼠肺组织病理学变化;酶联免疫吸附试验(ELISA)检测各组大鼠肺泡灌洗液中 IL-4、IL-5、IL-13、iNOS 含量;比色法检测大鼠肺组织中过氧化氢酶(CAT)活力及 H2 O2、NO 含量;免疫组化法检测大鼠肺组织中 P-p38 MAPK、p38 MAPK、P-IκBα、IκBα、P-NF-κB p65、NF-κB p65 蛋白的表达水平;实时荧光定量逆转录 PCR 法检测大鼠肺组织中 p38 MAPK、IκBα、NF-κB p65 mRNA 的表达水平.结果 与模型组相比,地塞米松组、艾灸组、无烟艾灸组肺组织病理形态均有不同程度的缓解;肺泡灌洗液中 IL-4、IL-5、IL-13 和 iNOS 含量显著降低(P<0.05,P<0.01);肺组织中 H2 O2、NO 含量显著降低(P<0.05,P<0.01)、CAT 含量显著升高(P<0.05,P<0.01);肺组织中 P-p38 MAPK、p38 MAPK、P-IκBα、NF-κB p65 和 P-NF-κB p65 蛋白表达量均显著降低(P<0.01),IκBα 蛋白表达量显著升高(P<0.01);肺组织中 p38 MAPK、NF-κB p65 mRNA 表达量均显著降低(P<0.05,P<0.01),IκBα mRNA 表达量显著升高(P<0.01).与地塞米松组比较,艾灸组与无烟艾灸组以上指标差异无统计学意义(P>0.05).与无烟艾灸组相比,艾灸组以上指标差异有统计学意义(P<0.05),表明艾烟在艾灸中起着不可缺少的作用.结论 艾灸及艾烟可能通过抑制 p38 MAPK/NF-κB 信号通路激活,改善 OVA 诱导的哮喘大鼠的炎症反应.
Objective To investigate the therapeutic mechanism of moxibustion and moxa smoke in alleviating airway inflammation in asthmatic rats through regulation of the p38 MAPK/NF-κB signaling pathway.Methods Male Sprague-Dawley rats were randomly assigned to seven groups:normal,model,dexamethasone,moxibustion,smokeless moxibustion,moxa smoke inhalation,and cigarette smoke inhalation.An asthma model was established by ovalbumin(OVA)sensitization and challenge.After successful modeling,rats in the dexamethasone group received intraperitoneal injection of dexamethasone sodium phosphate.The moxibustion group received moxibustion at bilateral Feishu(BL13),Dazhui(GV14),and bilateral Fengmen(BL12)acupoints for 30 min daily,while the smokeless moxibustion group underwent an identical procedure without smoke exposure.All treatments were administered once daily for 14 consecutive days.The model and normal groups received no intervention.Lung histopathological changes were observed.Bronchoalveolar lavage fluid(BALF)was collected to measure IL-4,IL-5,IL-13,and iNOS levels using enzyme-linked immunosorbent assay(ELISA).Catalase(CAT)activity and hydrogen peroxide(H2 O2)and nitric oxide(NO)contents in lung tissues were measured by colorimetric method.Protein expression levels of P-p38 MAPK,p38 MAPK,P-IκBα,IκBα,P-NF-κB p65,and NF-κB p65 in lung tissues were assessed using immunohistochemistry.mRNA expression levels of p38 MAPK,IκBα,and NF-κB p65 were quantified by reverse transcription quantitative real-time PCR.Results Compared with the model group,the dexamethasone,moxibustion,and smokeless moxibustion groups showed varying degrees of improvement in lung histopathology.BALF levels of IL-4,IL-5,IL-13,and iNOS were significantly reduced(P<0.05,P<0.01).H2 O2 and NO contents in lung tissues were markedly decreased(P<0.05,P<0.01),while CAT activity was significantly increased(P<0.05,P<0.01).Protein expression of P-p38 MAPK,p38 MAPK,P-IκBα,P-NF-κB p65,and NF-κB p65 was significantly downregulated(P<0.01),whereas IκBα protein expression was significantly upregulated(P<0.01).mRNA expression of p38 MAPK and NF-κB p65 was significantly decreased(P<0.05,P<0.01),and IκBα mRNA expression was significantly increased(P<0.01).No statistically significant differences were observed between the moxibustion or smokeless moxibustion groups and the dexamethasone group(P>0.05).However,significant differences were found between moxibustion group and smokeless moxibustion group(P<0.05),indicating that moxa smoke plays an essential role in the therapeutic effects of moxibustion.Conclusions Moxibustion and moxa smoke may alleviate the inflammatory response in OVA-induced asthmatic rats by inhibiting the activation of the p38 MAPK/NF-κB signaling pathway.
康璐;胡晓京;邵素菊;张雪琳
河南中医药大学,郑州 450046河南中医药大学第三附属医院,郑州 450008河南中医药大学,郑州 450046||河南中医药大学第三附属医院,郑州 450008河南中医药大学,郑州 450046||河南中医药大学第三附属医院,郑州 450008
医药卫生
哮喘艾灸艾烟气道炎症p38 MAPK/NF-κB信号通路
asthmamoxibustionmoxa smokeairway inflammationp38 MAPK/NF-κB signaling pathway
《中国比较医学杂志》 2026 (18)
1-12,12
国家自然科学基金面上项目(82074562)河南省科技攻关计划项目(242102310543)国家自然科学基金青年科学基金项目(81704174).
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