八仙花萼片开放过程中转录组分析及关键调控基因挖掘OA

The sepals of Hydrangea macrophylla are the primary ornamental structures.To investigate the molecular-level changes during H.macrophylla sepals development and identify the major molecular events,'Bailmer'and'Duro'were as materials.The differentially expressed genes across developmental stages were analyzed by transcriptome sequencing.The results revealed that stage-specific characteristics.In bud stage,cell wall and organelle reconstruction coupled with organic metabolism were primarily involved;in coloring stage,wax layer synthesis alongside fundamental metabolic processes were featured including signal transduction,ion transport,auxin transport and cellular development;in full-bloom stage,cellular maturation was marked,where basic metabolic processes were completed,shifting focus to photoreceptor activity,antioxidant responses,anthocyanin synthesis,chlorophyll degradation and carotenoid biosynthesis.The weighted correlation network analysis(WGCNA)identified critical transcription factors,including 7 transcription factors associated with cell expansion and auxin synthesis,2 transcription factors for wax synthesis,21 pigment-related transcription factors,and 10 transcription factors associated with chlorophyll degradation.Above results laid theoretical foundation for understanding the molecular mechanisms of sepal development in H.macrophylla.