基于DNA条形码与特征性成分的平纳鉴别研究OA

To address the challenge of accurately distinguishing Uyghur medicinal herb pingna(Mentha spicata)from its easily confused substitute,M.canadensis due to their morphological similarities,and to ensure medication safety,a multi-dimensional identification method was established in this study.Ten batches of pingna and five batches of M.canadensis samples were collected.DNA barcoding(ITS sequence)was used for molecular identification,combined with thin-layer chromatography(TLC)and gas chromatography(GC)for the qualitative and quantitative analysis of L-carvone,the characteristic component of M.spicata.The ITS phylogenetic tree clearly separated the two species into two distinct clusters.TLC results showed that all M.spicata samples contained the characteristic spot of L-carvone,which was absent in all M.canadensis samples.GC analysis confirmed that the L-carvone content in M.spicata ranged from 0.21 to 1.62 mg/g,while it was undetected in M.canadensis.The multidimensional identification system integrating DNA barcoding and chemical characteristic profiling established in this study enables rapid and accurate cross-verification of M.spicata and M.canadensis.This reliable method provides crucial technical support for quality control and standardization of M.spicata.