红树莓E2F/DP基因家族的鉴定及表达分析OA

The E2F/DP gene family plays a significant regulatory role in the growth,development and stress response of plants.The bioinformatics analysis of the E2F/DP gene family in red raspberry(Rubus idaeus)and the expression pattern analysis in different tissues and after exogenous spraying of melatonin lay the foundation for in-depth exploration of the function of E2F/DP genes in red raspberry.In this study,three members of the E2F/DP gene family were obtained from the transcriptome database of'Heritage'red raspberry.The E2F/DP gene family of red raspberry was analyzed by bioinformatics methods.Subsequently,the expression levels of E2F/DP in different tissues and organs,fruits at different developmental stages and fruits after exogenous spraying of melatonin were analyzed by qRT-PCR.The proteins encoded by RuE2F/DPs have a minimum of 243 amino acids and a maximum of 761 amino acids,with a minimum relative molecular mass of 26.88 kDa and a maximum of 82.55 kDa.Their isoelectric points range from 5.69 to 9.07.RuE2F/DP-1 is a stable hydrophilic protein,while RuE2F/DP2-3 is unstable hydrophilic protein.All RuE2F/DPs-encoded proteins are located in the cell nucleus,have no signal peptides,are non-secretory proteins,non-transmembrane proteins,and contain the conserved E2F_TDP domain.Their secondary structures are all composed of four types of structures,including α-helices,β-turns,random coils,and extended strands.The prediction results of their tertiary structures are consistent with their secondary structures.RuE2F/DP-1 and RuE2F/DP-3 have expression correlations.The RuE2F/DP gene family contains 9 to 14 exons and 4 to 10 conserved motifs;phylogenetic analysis indicates that the RuE2F/DP family members belong to different subgroups,and red raspberry is more closely related to strawberry;the cis-acting elements in the promoter suggest that the RuE2F/DP gene family mainly participates in plant growth and development and responses to drought,low temperature and other stress environments;qRT-PCR analysis shows that the expression levels of three members of the RuE2F/DP gene family in young roots and green fruits are higher than those in other tissues;melatonin treatment has a positive regulatory function on RuE2F/DP1-2 and a negative regulatory function on RuE2F/DP-3.The research results reveal that the RuE2F/DP gene family may play a role in the growth of red raspberry roots,the growth and development of fruits,the response to adverse stress during the growth process,and the hormone response to melatonin.