不同载体介导的RNAi在鳞翅目昆虫防控中的应用研究进展OA

To address the bottlenecks in RNAi-based control of Lepidoptera insects,such as nuclease degradation and low delivery efficiency due to the alkaline gut environment,this study systematically reviews the current applications and potential of different delivery carriers for mediating RNAi.Using a literature analysis approach,we summarize the structural characteristics,delivery mechanisms,and existing challenges in the biological control of lepidopteran pests of carriers such as bacteria,viruses,liposomes,and polymers.The advantages and limitations of each carrier in mediating RNAi for Lepidoptera insects are compared.The results indicate that live bacterial carriers are low-cost and can achieve up to 68%mortality in Plutella xylostella;viral carriers offer high transfection efficiency and protect RNA from degradation by midgut nucleases;liposomal carriers enhance cellular internalization via electrostatic interactions,increasing mortality in Chilo suppressalis to 32%;and polymer carriers(e.g.,chitosan)exhibit strong stability,with spray application resulting in 100%mortality in Helicoverpa armigera and remaining active on leaf surfaces for up to 5 days.Through analysis,it is determined that various carriers enhance RNAi efficiency by preserving RNA integrity and improving cellular uptake.Among them,polymer-based carriers and biological carriers(e.g.,endophytic and symbiotic bacteria)demonstrate outstanding potential for field applications.This study provides a carrier selection reference for green control of Lepidoptera pests and offers technical insights for the commercial development of RNAi-based bio-pesticides.