工业大麻籽实脂肪酸和蛋白质的动态变化分析OA

The study aims to learn the dynamic changes of fatty acid and protein accumulation during the maturation process of industrial hemp seeds,and to explore the interaction of related enzymes,fatty acids,proteins and other related components during its development.Taking industrial hemp variety'Geliexiya'as the research object,the seeds were sampled at different stages of 6 days(S1),12 days(S2),18 days(S3)and 21 days(S4)after flowering,and the moisture content,hundred-grain weight,fatty acid content,protein content,FAS activity and GS activity were determined and analyzed.The results showed that the moisture content of industrial hemp seeds increased in the period of S1-S2 and decreased in the period of S2-S4;the hundred-grain weight increased in the period of S1-S4;the fatty acid content decreased in the period of S1-S2 and increased in the period of S2-S4;the protein content decreased in the period of S1-S2 and increased in the period of S2-S4;the FAS and LOX were significantly negatively correlated with the fatty acid content;NR,GS,GDH,and GOGAT were highly significantly positively correlated with protein.Among them,FAS showed the highest correlation with fatty acid content,and GS showed the highest correlation with protein content.The conclusion is that fatty acids and proteins in industrial hemp seeds are synergistically accumulated in the middle and late stages of maturation,and FAS and GS are the core enzymes that regulate the accumulation of the two.This study provides a theoretical basis for the breeding of new varieties of high oil and high protein industrial hemp and the optimization of cultivation techniques.In the future,the molecular regulation mechanism can be further analyzed in combination with multi-omics technology.