重组Ⅲ型胶原蛋白与重组弹性蛋白注射对紫外线致裸鼠皮肤急性光损伤的修复作用研究OA

Objective To investigate the repair effects of recombinant type Ⅲ collagen(rCol Ⅲ)and recombinant elastin(rEln)injection on the repair of acute photodamage of nude mouse skin induced by ultravioletlight.Methods Twenty-five female BALB/c-Nude mice were randomly divided into five groups(n=5 per group):Group A was the blank control group;Group B was the group with photodamage,Group C was the photodamage plus normal saline injection group(Normal Saline,NS),Group D was the photodamage plus recombinant type Ⅲ collagen injection group(rCol Ⅲ);Group E was the photodamage plus recombinant elastin injection group(rEln).We used a UVB dose of 1 000 mJ/cm²,at a distance of 30 cm,irradiated for 60 minutes/day for 8 days.Group A didn't receive any treatment,Group B was subjected to UVB irradiation to induce acute photodamage,and Groups C,D,and E received subcutaneous injections of 0.1 ml NS,rCol Ⅲ(1.0 mg/ml),and rEln(1.0 mg/ml)on the fourth day after UV irradiation.Gross observation was used to assess skin appearance changes.Pathological alterations were evaluated by hematoxylin-eosin(HE)staining and Masson staining.Reactive oxygen species(ROS)levels were detected via immunofluorescence.Immunohistochemical staining was used to detect the expression rate of proliferating cell nuclear antigen(PCNA)and the percentage of positive cells for matrix metalloproteinase-1(MMP-1).Results Group B showed obvious skin scales,decreased luster,local redness,ulceration,and crust formation.These lesions were significantly alleviated in Groups D and E,with milder overall severity compared to Group B.HE staining showed varying degrees of epidermal thickening,reduced skin appendages,inflammatory cell infiltration,and capillary dilation in all groups except Group A.The pathological manifestations in Groups D and E were milder,and the measured epidermal thickness was significantly lower than that in Group B(P＜0.05).Masson staining indicated that collagen fibers(blue-stained)in Groups B and C were reduced,unevenly distributed,and discontinuous,while these abnormalities were improved in Groups D and E.ROS detection showed that the average fluorescence intensity in Group B was significantly higher than that in Group A(P＜0.05),and significantly lower in Groups D and E than in Group B(P＜0.05).Immunohistochemical results showed that PCNA expression in Group B was significantly lower than that in Group A(P＜0.05),while MMP-1 positive cells were significantly higher(P＜0.05).Compared with Groups B and C,Groups D and E had significantly higher PCNA expression and lower MMP-1 positive cells(P＜0.05).Conclusion Injections of recombinant type Ⅲ collagen and recombinant elastin exert significant reparative effects on acute ultraviolet-induced photodamage in nude mouse skin.