铁皮石斛花外泌体样纳米颗粒的制备及体外抗肿瘤作用OA

Objective To isolate and prepare extracellular vesicle-like nanoparticles from Tiepishihu(Dendrobium officinale)flowers(DF-EVLPs),and evaluate their specific targeting ability for colon cancer cells,as well as investigate their in vitro targeting and anti-tumor effects against colon cancer cells,in order to provide experimental evidence for the development of targeted delivery systems based on medicinal and edible plants.Methods DF-EVLPs were isolated fromD.officinale flowers and their morphology and particle size were characterized.The uptake of DF-EVLPs by CT26 colon cancer cells was observed using pKH26 fluorescence labeling.The effects of DF-EVLPs on the proliferation,migration and apoptosis of CT26 cells and their mechanism were evaluated using CCK-8 assay,scratch wound healing experiment,flow cytometry and Western blotting.Results The obtained DF-EVLPs showed a uniform cup-shaped morphology with an average particle size of(156.9±7.2)nm.The cell uptake experiment demonstrated that DF-EVLPs could be efficiently internalized by CT26 colon cancer cells,and the uptake efficiency of DF-EVLPs for colon cancer cells was significantly higher than that of normal colonic epithelial cells,indicating their good specific targeting ability.The in vitro functional experiments showed that DF-EVLPs inhibited the proliferation of CT26 cells in a concentration-dependent manner,significantly reduced cell migration ability,and induced cell apoptosis.Conclusion This study successfully prepared extracellular vesicle-like nanoparticles from D.officinale flowers.As a new type of food-derived nanotherapeutic agent,DF-EVLPs can specifically target colon cancer cells and exert in vitro anti-tumor activity.This study reveals a new biological activity function of D.officinale flowers and provides experimental evidence for dietary intervention for colorectal cancer based on the"medicinal and edible"concept.